摘要
目的：建立一种改良的兔晶状体上皮细胞体外培养的方法，提高晶体上皮细胞原代培养的成功率。方法：利用改良消化法对兔晶体上皮细胞进原代培养，对培养的细胞进行形态学观察。结果：接种48小时～72小时后即可见上皮细胞贴壁生长，具备上皮细胞的形态特点；约7天后融合。传至5代后，细胞明显成纤维细胞化。结论：此种方法经济简便，可为各种实验提供兔晶体上皮细胞。
Objective： To establish a developed procedure to culture rabbit lens epithelial cells for increasing the success rate of its primary culture. Methods： Using the developed procedure to culture rabbit lens epithelial cells, and the cultured cells were examined morphologically. Result： Primary culture for 48 to 72 hours, the lens epithelial grew, they maintained typical epithelial morphology and reach confluence after about 7 days. After passage 5 , the morphology of cells was transformed to fibroblast apparently. Conclusion： This technique is simple and lower cost to provide rabbit lens epithelial cells for use in various experiments.
出处
《中国伤残医学》
2006年第2期23-25,共3页
Chinese Journal of Trauma and Disability Medicine
关键词
晶体上皮细胞
细胞培养
晶状体
lens epithelial cell cell culture lens crystalline
