摘要
对615小鼠可移植性白血病瘤株L_(7712)及其用更生霉素化疗后建立的L_(797)瘤株进行了染色体核型、电镜、组化等多方面的分析比较。结果发现L_(797)干系细胞众数及染色体核型与L_(7712)有很大不同,但两瘤株皆具有一个共同的高度特异标记染色体,电镜、组化资料提示L_(797)仍属于T淋巴细胞白血病。实验还发现更生霉素有很强的致615小鼠骨髓细胞染色体畸变作用。初步认为L_(797)是一株来自L_(7712)又不同于L_(7712)的变异瘤株。其变异机制很可能是肿瘤细胞遗传物质的不稳定性及更生霉素对DNA的损伤造成了染色体重排,形成了新的细胞遗传学特征,逃脱了化疗药物的杀伤而成瘤。
The karyotype, ultrastructure, histochemistry and morphology assayof the 615 mouse transplantable leukemia L_(797) have been studied andcompared with those of L_(7712). L_(797) was formed after the Dactinomycin(DACT) chemiotherapy on L_(7712). The results show that the modelnumber and karyotypes of L_(797) are quite different from those of L_(7712).But significantly, both tumor cell lines have a same marker chromosomewith peculiar bands. The observations of ultrastructure, histochemistryand morphology also support L_(797) belongs to the same type of leukemia,T-lymphocyte leukemia, as L_(7712). Therapeutic dorsage of DACT wasused on normal 615 mouse, the result shows that DACT has strongeffects on causing chromosome aberration of the cells. The evidencesindicate that L_(797) stemed, but differed from L_(7712) The possiblemechanism of the tumor cell transformationis most likely the DNAdamage effect of DACT and the unstability of tumor cell's hereditarysubstances, causing the rearrangement of chromosomes and creating thenew phenotypes of tumor cells. Some of the new type tumor cells havethe ability to escape from the killing action of DACT, thus a newtumor cell line was developed.
关键词
白血病
核型分析
化疗
小鼠
mouse leukemia
karyotype analysis
chemotherapy
