摘要
背景:研究表明,针灸治疗有一定作用,三焦经是临床治疗耳病的首选穴位,但其机制尚不十分清楚。目的:观察针刺三焦经腧穴翳风、耳门、中渚穴对庆大霉素中毒性听力损害的改善作用。设计:完全随机分组设计。单位:北京首都医科大学宣武医院神经内科。材料:选用健康白色雄性豚鼠60只,3～4月龄,普通级,体质量250～300g,耳廓反射正常。按随机抽签法将鼠分为5组:正常组、模型组、翳风穴组、耳门穴组、中渚穴组,每组12只。方法:实验于2001-01/07在北京中医药大学针灸实验室完成。①正常组保持正常饲养条件,模型组、针刺各组每日肌肉注射庆大霉素80mg/kg,连续20d。3个针刺组分别选取翳风、耳门、中渚穴,在给药同时两耳分别进行针刺治疗,针刺前豚鼠固定,选用0.35mm×25mm不锈钢毫针,快速进针刺入皮下,直刺约10mm,平补平泻捻转行针,隔10min1次,0.5min/次,留针30min,治疗20d。②干预前后采用7S11A诱发电位仪进行脑干听觉反应测试,声源通过耳机输入,检测豚鼠脑干听觉反应阈值。③组间计量资料差异比较采用t检验。主要观察指标:针刺翳风、耳门、中渚穴对庆大霉素致听力损害大鼠脑干听觉反应阈值的影响。结果:豚鼠60只均进入结果分析。干预前,各组间脑干听觉反应阈值差异不明显(P>0.05);干预后,模型组、翳风组、耳门组、中渚组鼠脑干听觉反应阈值明显高于正常组(P<0.01),翳风组、耳门组、中渚组鼠脑干听觉反应阈值明显低于模型组(P<0.01),其中,翳风组与模型组差异最明显。结论:针刺耳门、中渚穴,特别是翳风穴可以减轻庆大霉素耳毒性。
BACKGROUND： Some researches indicated that the acupuncture treatment, especially at Sartjiao meridian might be effective for otopathy. However, the mechanism is not clear yet. OBJECTIVE： To observe the hearing improvement of the cavies induced by gentamicin after the animals received the acupuncture at Sanjiao meridian acupoints： Yifeng （SJ 17）, Ermen （SJ 21） and Zhongzhu （SJ 3）. DESIGN： Complete randomization. SETTING： Department of Neurology, Xuanwu Hospital, Capital University of Medical Sciences. MATERIALS： Sixty healthy white male cavies of 3-4 months old, common grade, 250-300 g weight and normal auricle reflex were selected and randomly arranged into 5 groups： normal group, model group, acupuncture groups of Yifeng （SJ 17）, Ermen （SJ 2 1） and Zhongzhu （SJ 3）, with 12 ones in each. METHODS： The experiment was conducted at the Laboratory of Acupuncture and Moxibustion, Beijing University of Chinese Medicine from January to July in 2001.①The normal cavies were bred at normal conditions, while other cavies were injected intramuscularly with 80 mg/kg gentamicin every day, continuously for 20 days. At the same time of gentamicin injection, Yifeng （SJ 17）, Ermen （SJ 21） and Zhonzhu （SJ 3） were selected for acupuncture treatment in the corresponding groups. The fixed cavies were pricked subcutaneously with 0.35 mm×25 mm stainless steel acupuncture needles for 10 mm straight depth. The needles were twisted with mild reinforcing-reducing method once in half a minute, repeated every 10 minutes, and remained for 30 minutes one day for 20 days.②Before and after the intervention, auditory brain-stem response （ABR） was detected with 7S11A evoked potential apparatus, in which the sound source was input by earphone for measuring the threshold value of ABR in cavies. ③The difference of measurement data were compared with t test between groups. MAIN OUTCOME MEASURES： The effect of acupuncture treatment at Yifeng （SJ 17）, Ermen （SJ 21） and Zhong：hu （
出处
《中国临床康复》
CSCD
北大核心
2006年第31期159-161,共3页
Chinese Journal of Clinical Rehabilitation
