摘要
目的:通过测定糖耐量变化,分析荔枝核提取液对四氧嘧啶糖尿病模型小鼠生化指标的干预作用。方法:实验于2005-12/2006-01在右江民族医学院生化教研室完成。①荔枝核,为广西百色市靖西县产酸荔枝果核,晒干后粉碎,水提取,加热煮沸30min后,过滤去渣浓缩,加乙醇沉淀,再过滤去沉淀,加热挥发乙醇,提取,得到含浓度1.2g/mL生药水提液。②选取清洁级健康昆明种小白鼠40只,分为正常对照组、模型对照组、荔枝核提取液低浓度组、荔枝核提取液高浓度组,10只/组。③除正常对照组外,其余各组均按200mg/kg体质量腹腔注射四氧嘧啶水溶液建立糖尿病模型。48h后测血糖,血糖未升高的小鼠按100mg/kg腹腔注射四氧嘧啶水溶液,连续追加2次。③待空腹血糖升高和糖耐量异常后,荔枝核提取液低浓度组用荔枝核水提取液给予灌胃治疗,每只小鼠剂量为0.5mL(内含0.012g荔枝核生药);荔枝核提取液高浓度组每只小鼠剂量为0.5mL(内含0.024g荔枝核生药);正常对照组、模型对照组给予等量蒸馏水灌胃。1次/d,连续7d。④测定造模前后空腹血糖,治疗前后糖耐量血糖,治疗后血清丙氨酸氨基转移酶、尿素、三酰甘油、总胆固醇含量。制备大脑匀浆,测定乙酰胆碱酯酶活性、超氧阴离子自由基(O2-)浓度和清除率、蛋白含量。结果:实验选取健康昆明种小白鼠40只,全部进入结果分析。①造模前后各组小鼠血糖含量的变化:与造模前比较,造模后2,6,9d模型对照组、荔枝核提取液低浓度组、荔枝核提取液高浓度组血糖含量均显著升高,而正常组无明显变化。②治疗前后各组糖耐量试验血糖含量的比较:治疗前30min,与正常对照组比较,其余3组血糖含量均明显升高(P<0.05或P<0.01),显示耐糖量较差。治疗后30min,与正常对照组比较,其余3组血糖含量亦均明显升高(P<0.05或P<0.01)。治疗后2h除模型对照组外,荔枝核提取
AIM. To explore the intervention of litchi nucleus extract fluid on blood biochemical indexes of mice models with diabetes mellitus induced by alloxan.
METHODS： The experiment was carried out in the Biochemical Institute, Youjiang Medical College of Nationalities between December 2005 and January 2006. ①The extract fluid containing 1.2 g/mL crude drug was prepared with the sour litchi nucleus from Jingxi County, Baise City of Guangxi, shattered after drying and extracted with water, followed by filtering and concentrating after 30 minutes of heating to precipitate with alcohol, and filtered again to remove the precipitate, and heated to volatilize the alcohol. ②Forty clean healthy Kunming mice were selected and divided into 4 groups： Control group, model group, low concentration extract fluid group and high concentration extract fluid group with 10 mice in each group. ③Except for the control group, all the rest mice were intraperitoneally injected with alloxan liquid to establish the diabetes models. The blood glucose was measured 48 hours later, those whose blood glucose was not increased were reinjected the alloxan for successive twice by body mass of 100 mg/kg. ④When the fasting blood glucose （FBG） increased and the sugar tolerance was abnormal, the litchi nucleus extract fluid with dose of 0.5 mL containing 0.012 g and 0,024 crude drug was infused into the stomachs of mice of the low and high concentration groups, respectively; While the mice in the control and model groups were given the same dose of distilled water. The administration was given once a day for 7 days.⑤ FBG before and after establishment of models, sugar tolerance before and after treatment, alanine aminotransferase （AAT） in blood, urea, triglyceride （TG） and total cholesterol （TC） after treatment were determined. The acetylcholine esterase （Ache） activity, density and clearance of superoxide radical anion （O2^-） and the protein content were determined after preparation of homogenized brain.
RESULTS�
出处
《中国临床康复》
CSCD
北大核心
2006年第31期61-63,共3页
Chinese Journal of Clinical Rehabilitation
