摘要
目的：了解ｐ５３基因杂合性丢失与胃癌发生、发展的关系。方法：应用位于１７ｐ１３区域的ｐ５３ｃＤＮＡ探针和ｐＹＮＺ２２ＶＮＴＲ探针，对胃癌及癌旁正常组织基因组ＤＮＡ进行Ｓｏｕｔｈｅｒｎ杂交。结果：在２２例胃癌中，１２例正常组织被检出有杂合性，其中６例标本癌组织发生了杂合性丢失，检出率为５０％。
OBJECTIVE:To investigate the relationship between loss of heterozygosity(LOH) of p53 and development of human gastric cancer.METHODS:Southern blot by p53 cDNA and pYNZ22 VNTR probes located in 17p13 region were employed to detect the DNA of paired gastric tumor and normal tissues.RESULTS:The LOH of p53 gene was found in 6 of 12 cases of gastric cancer.The rate of LOH was 50% (6/12).CONCLUSION:LOH of p53 gene is a frequent event in the development of human gastric cancers.
出处
《南京铁道医学院学报》
1996年第3期147-149,共3页
Journal of Nanjing Railway Medical College
基金
江苏省自然科学基金
关键词
胃癌
P53基因
杂合性丢失
病理
gastric cancer
p53 gene
loss of heterozygosity (LOH)
