摘要
为了给内脏利什曼病（ＶＬ）患者提供较为准确的实验诊断方法。我们将表达杜氏利什曼原虫３９ｋＤ抗原的大肠杆菌制备物进行电泳并转移至硝酸纤维滤膜上，以１１例确诊ＶＬ病人的不同稀释度血清对其识别，当稀释度达１∶４００时，全部病人血清均能明显识别３９ｋＤ抗原带．而正常人血清和其他病人血清不识别３９ｋＤ抗原带，说明重组杜氏利什曼原虫３９ｋＤ抗原具有一定的诊断价值，用其检测病人血清中相应抗体可以诊断内脏利什曼病。
In order to develop the application potential of the recombinant 39kD antigen of Leishmania donovani for the detection of anti-leishmania antibodies in human serum samples, the E. coli Y1189r+ extracts ahich contained the recombinant 39kD antigen of Leishmania donovani were identfied by different dilution titers of the serum samples from 11 patients with parasitologically proved visceral leishmaniasis (VL) using Western-blot. With a cutoff value of 1: 400, the extracts remained full active even after storage at -70℃ for 5 years. Allowed reproducible results in the immunoblotting for same serum samples over a long Period of time, as was shown, make the recombinant 39kD antigen a valuable candidate for serological diagnose of visceral leishmaniasis.
出处
《实用寄生虫病杂志》
CAS
1996年第3期97-99,共3页
Journal of Practical Parasitic Diseases
基金
四川省科学技术委员会科研基金
关键词
杜氏利什曼原虫
重组抗原
诊断价值
Leishmania donovani, recombinant 39kD antigen, diagnostic value
