摘要
目的建立并分析人鼻腔NK／T细胞淋巴瘤的基因表达谱。方法鼻腔NK／T细胞淋巴瘤组织和正常淋巴结组织各6例,分别进行总RNA的提取,通过逆转录过程获得标记有荧光的cDNA,分别与2张相同的包含有4096条基因的基因芯片进行杂交,根据杂交结果筛选出与鼻腔NK／T细胞淋巴瘤相关的基因。结果2张芯片中,差异表达趋势一致并且表达强度均相差1倍以上的基因共有365条(8．9％),包括癌基因和抑癌基因,细胞周期相关基因,细胞凋亡相关基因,DNA合成、修复和重组相关基因,DNA结合、转录和转录因子基因,细胞信号传递相关基因,细胞代谢相关基因以及蛋白翻译、合成相关基因等,其中37条基因差异表达4倍以上。结论鼻腔NK／T细胞淋巴瘤有多条差异表达基因,初步建立了其基因表达谱。
Objective To investigate the changes of gene expression profile in nasal NK/T cell lymphoma. Methods Total RNA was extracted from the fresh nasal NK/T cell lymphoma tissue and normal lymph node. Fluorescent labeled cDNA was obtained through synthesizing process by reverse transcription. After hybridization in the two identical microarrays consisting of 4096 genes, overexpressed or underexpressed tumor related genes were analyzed. Results In both experimental group and control group, there were six samples. A total of 365 （8.9%） genes was found to be differentially expressed by a factor of twofold or greater in both of two identical cDNA microarrays, which included oncogenes, tumor supressor genes, cell cycle regulators, apoptotic and antiapeptotic factors, DNA transcription factors, DNA repair and recombination factors, signal transduction genes, protein translation genes, as well as a large number of metabolic genes. Thirty-seven of these genes were found to be differentially expressed by a factor of fourfold or greater. The biochemical functions of these differentially expressed genes were diverse. Conclusion This study demonstrates that many different kinds of genes are possibly involved in the initiation and progression of nasal NK/T lymphoma, eDNA microarray technique is useful in screening cancer gene expression for nasal NK/T lympboma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2006年第7期518-522,共5页
Chinese Journal of Oncology
