摘要
目的观察金消方对小鼠Lewis肺癌增殖及凋亡的影响。方法50只小鼠复制Lewis肺癌小鼠模型,随机分为5组:空白对照组、单纯化疗组、金消方高剂量组、金消方低剂量组和金消方化疗组各10只。空白对照组给予生理盐水腹腔注射;单纯化疗组给予顺铂1mg/kg腹腔注射;金消方高、低剂量组分别给予金消方25g/kg和12.5g/kg灌胃;金消方化疗组给予金消方25g/kg灌胃+顺铂1mg/kg隔日腹腔注射。15天后采用免疫组化及流式细胞检测方法,对抑瘤率、增殖细胞核抗原(PCNA)指数及凋亡率进行检测。结果金消方化疗组抑瘤率及凋亡率明显高于单纯化疗组和金消方高、低剂量组,PCNA明显降低(P<0.05)。金消方高、低剂量组与单纯化疗组比较,抑瘤率、凋亡率及PCNA均较低(P<0.05)。结论诱导细胞凋亡、抑制肿瘤细胞增殖可能是金消方治疗肺癌的分子机制之一,与化疗药合用有协同增效作用。
Objective To investigate the effect of Jinxiaofang on proliferation and apoptosis in mice with Lewis lung cancer. Methods The Lewis lung cancer models were made in 50 mice and were randomly divided into five groups： normal group, control group, high-dose Jinxiaofang group, low-dose Jinxiaofang group and treatment group. The normal group were given saline by intraperitoneal injection, the control group were given cisplatin 1 mg/kg by intraperitoneal injection, the high-dose Jinxiaofang group and low-dose Jinxiaofang group were given Jinxiaofang 25 g/kg and 12.5 g/kg by gavage respectively,the treatment group were given Jinxiaofang 25 g/kg and cisplatin 1 mg/kg by intraperitoneal injection every other day. After 15-day＇ s treatment. The inhibitory tumor rate, proliferating cell nuclear antigen （PCNA） index and apoptotic rate were detected by immunohistochemistry and flow cytometry. Results The inhibitory tumor rate and apoptotic rate in treatment group were significantly higher than those of control group, high-dose Jinxiaofang group and low-dose Jinxiaofang group （ P 〈 0.05）, however, PCNA index in treatment group was significantly lower（ P 〈 0.05）. Compared with control group, the inhibitory tumor rate, apopototic rate and PCNA index in high-dose Jinxiaofang group and low-dose Jinxiaofang group were lower （ P 〈 0.05）. Conclusion Jinxiaofang can significantly induce cell apoptosis, inhibit the proliferation of tumor cells, which may be one of the antineoplastic mechanisms. Jinxiaofang combined with chemotherapeutic drugs may increase therapeutic effect.
出处
《疑难病杂志》
CAS
2006年第4期244-246,共3页
Chinese Journal of Difficult and Complicated Cases
