摘要
目的:观察丁酸钠诱导培养的乳腺癌细胞MCF-7凋亡的影响。方法:用终浓度分别为0、1.25、2.5、5.0、10.0mmol/L的丁酸钠处理MCF-7细胞48h,用MTT比色法分析丁酸钠对细胞的抑制率,倒置显微镜观察细胞生长情况的改变,流式细胞术,末端脱氧核糖核酸转移酶介导的缺口末端标记法(TUNEL)及DNA琼脂糖凝胶电泳检测凋亡。结果:不同浓度的丁酸钠对MCF-7细胞有明显的抑制作用,光镜下观察到细胞贴壁性随作用时间延长而下降;流式细胞术检测不同浓度丁酸钠处理细胞的凋亡率分别为4.8%、9.4%、26.1%和51.9%;TUNEL检测可见阳性细胞胞体缩小,核固缩,呈棕黄色或黄褐色颗粒;DNA琼脂糖凝胶电泳检测到10mmol/L处理细胞出现“DNA ladders”。结论:丁酸钠可以诱导MCF-7细胞发生凋亡。
Objective：To observe the influence of the apoptosis of cultured MCF-7 cell induced by sodium butyrate. Methods： MCF-7 cells were treated with the final concentrations 0,1.25,2.5,5.0,10.0mmoL/L of sodium butyrate for 48h, and analyzed the inhibition ratio of sodium butyrate on cells with MTT. The changes of cell growth were observed under inverted microscope. Flow cytometry, terminal-deoxynucleotidyl transferase mediated nick end labeling（TUNEL） and agarose gel electrophoresis were used to analyze the apoptosis of cells. Results： Different concentrations of sodium butyrate can inhibit the growth of MCF-7 cells evidently. The cells adherence decreased as increasing of time of treatment under microscope. The cell apoptosis rates were 4.8 % , 9.4 %, 26.1% and 51.9 % by treatment of different concentrations of sodium butyrate. Some cell bodies were deflated and nucleuses were karyopyknosis and yellow or yellow brown granules. “DNA ladders” was found on agarose gel electrophoresis from cells DNA under the concentration of 10mmol/1. Conclusion： Sodium butyrate can induce MCF-7 cells apoptosis.
出处
《现代肿瘤医学》
CAS
2006年第8期916-918,共3页
Journal of Modern Oncology
基金
广东省重点扶持学科资助(课题号:9808)
关键词
丁酸钠
乳腺癌细胞
凋亡
sodium butyrate
breast cancer
apoptosis
