摘要
采用烟碱为唯一碳源的选择性培养基,对烟草、植烟土壤及烟草污水中的细菌进行选择性分离,共分离得到97株能够分解利用烟碱的细菌,其中菌株K9分解能力最强。经生理生化分析以及16SrDNA序列同源性分析,鉴定为烟草节杆菌(Arthrobacternicotianae)。为微生物降烟碱的研究及应用提供了理论依据。
97 bacterial strains were separated and screened from tobacco,soil and sewage of tobacco factory with selectivity culture medium, in which nicotine was the sole carbon source. These strains could decompose and utilize nicotine,and strain K9 was best one that have strongly ability to degrade nicotine. Based on physiology and biochemistry characters and 16S rRNA gene sequence homology, the strain K9 was identified as A rthrobacter nicotianae. This study lays the foundation for the application of nicotine biodegradation.
出处
《中国烟草科学》
CSCD
2006年第2期1-4,共4页
Chinese Tobacco Science
基金
国家自然科学基金资助项目(30460007)
云南省烟草公司科技计划项目(04A17)
关键词
烟碱
生物降解
16S
rDNA鉴定
烟草节杆菌
nicotine
biodegradation
16S rDNA
A rthrobacter nicotianae
identification
