摘要
以淡水经济微藻雨生红球藻(HaematococcuspluvialisCH-1)为试验藻种,分别添加维生素B1(VB1)、B12(VB12)和维生素H(VH),每种维生素各分6个浓度梯度,测定了雨生红球藻的细胞密度、生物量、叶绿素a和类胡萝卜素含量等指标。结果表明,分别添加维生素B1、B12和H均显著促进了雨生红球藻细胞的生长。维生素B1、B12和H对雨生红球藻的最佳添加浓度分别为10mg·L-1、50μg·L-1和500μg·L-1。在维生素B1、B12和H各自的最佳浓度处理下,雨生红球藻的细胞密度、生物量、叶绿素a和类胡萝卜素含量等4项指标均比对照有显著提高:维生素B1处理分别提高了20.1%、14.6%、23.2%和21.3%;维生素B12处理分别提高了29.5%、30.0%、28.0%和24.4%;维生素H处理分别提高了17.1%、29.2%、21.8%和10.1%。在雨生红球藻规模化生产的游动细胞培养阶段,适当地添加维生素B1、B12和H均能够有效地提高藻细胞密度、生物量、叶绿素a和类胡萝卜素含量。
An economic microalgae Haematococcus pluvialis CH-1 was used as experimental material. An experiment of adding six grades of concentrations of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively was conducted. Cell density, biomass, chlorophyll-a, and carotenoid content were measured. The results showed that the growth of H. pluvialis was accelerated significantly by adding of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively. The optimal adding concentration of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively for H. pluvialis was 10 mg·L^-1, 50μg·L^-1, and 500μg·L^-1. Under the optimal concentration of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively for H. pluvialis, cells density, biomass, chlorophyll-a and carotenoid content were enhanced with Vitamin-B1： 20.1%, 14.6%, 23.2% and 21.3%; Vitamin-B12： 29.5%, 30.0%, 28.0% and 24.4%; Vitamin-H： 17.1%, 29.2%, 21.8% and 10.1% higher than the control respectively. On the mass culture of motile cells of Haematococcus pluvialis, properly adding Vitamin-B1, Vitamin-B12 and Vitamin-H respectively was effective for increase cells density, biomass, chlorophyll-a and carotenoid content.
出处
《生态科学》
CSCD
2006年第2期102-105,共4页
Ecological Science
基金
国家自然科学基金(30370231)
珠海市科技计划重大项目(PB20041018)
广东省科技计划重点引导项目(2005B33201001)
