摘要
目的研究高浓度二甲基亚砜(DMSO)和60Co辐照对韧带移植物的力学特性和超微结构的影响。方法取兔髌韧带移植物,分别作新鲜冰冻和65%DMSO处理。采用0 Mrad2、.5 Mrad和5 Mrad剂量60Co分别辐照2组移植物后,作组织学、超微结构和生物力学检测。结果组织学检查未发现明显差异。超微结构中0 Mrad辐照后DMSO组髌腱移植物胶原结构与正常髌腱无明显区别而新鲜冰冻组胶原直径明显增大,胶原间隙明显增大,小直径胶原比例下降。2.5 Mrad剂量60Co辐照下两组间超微结构形态没有显著差异。5 Mrad剂量60Co辐照下2组都出现明显的胶原碎裂表现。在新鲜冰冻和65%DMSO处理组,5 Mrad辐照后移植物强度都较采用0 Mrad和2.5 Mrad辐照有显著下降。结论高剂量辐照会造成韧带移植物强度明显下降,高浓度DMSO对辐照状态下韧带基质胶原没有明显保护作用。
Objective To investigate biomechanical and ultrastructural properties of tendon allografts which were treated by high concentration dimethylsulfoxide and 60Co irradiation. Methods Thirty- six patellar tendon allografts of rabbits were divided into two groups which were treated by 65 % dimethylsulfoxide （DMFO） before deep frozen or only fresh frozen. Histological finding, ultrastructural and biomechanical characteristics were respectively tested after iradiation with 60Co of 0 Mrad, 2.5 Mrad and 5 Mrad in two groups. Results There were no differences between the two groups in histological finding. The collagen ultrastructure of grafts treated by 65 % DMSO without irradiation were similar with that of normal patellar tendons. But in deep frozen group, larger diameter collagen fibrils and greater interfibrillar space appeared and the quantity of small diameter collagen fibrils decreased. No obvious differences were found between two groups with 2.5 Mrad irradiation. Chip appearance of collagen occurred in both two groups with 5 Mrad irradiation. Compared with that of other two groups, biomechanical strength of grafts treated with 5 Mrad irradiation was significant decreased. Conclusion High dose irradiation will lead to decrease of biomechanical strength for tendon grafts. With 65% dimethylsulfoxide, there was no irradiated protection for collagen structure of tendon allografts.
出处
《新乡医学院学报》
CAS
2006年第4期350-352,F0003,共4页
Journal of Xinxiang Medical University
基金
上海市卫生局青年基金项目
课题编号:034Y09
