摘要
目的比较酒精以及酒精与二硝基氯苯、卡介苗、雄黄的不同组合液对B16移植性黑色素瘤小鼠瘤内注射的疗效。方法由B16细胞接种产生的移植性黑色素瘤C57BL／6小鼠30只，随机分5组，每组6只，每鼠分别以0．1mL的纯酒精（纯酒组），酒精与卡介苗混悬液（卡酒组，卡介苗15μg），酒精与二硝基氯苯（DNCB）溶液（D酒组，DNCB1mg），酒精与雄黄混悬液（雄酒组，雄黄1mg），以及磷酸钠（PBS）缓冲液（对照组）局部瘤内注射，每周注射2次。每10d观察各组肿瘤大小，并于d30杀鼠剥离肿瘤称重。结果D酒组6鼠中有1鼠，于第3次注射后20h死亡。接种30d后，纯酒组、卡酒组、D酒组、雄酒组及对照组的瘤重分别为（2．19±1．69）、（1．76±1．32）、（0．13±0．10）、（2．61±1．45）以及（3．97±1．01）g，D酒组与其它各组间均有显著差别（P〈0．05）。结论很低剂量（1mg）的半抗原DNCB与纯酒精的溶液瘤内注射治疗移植性黑色素瘤小鼠，可明显抑制肿瘤生长，对PBS及纯酒精的相对抑瘤率分别高达96．73％及94．06％，有临床应用的潜力。
Objective To compare effect of intra-tumor injection of ethanol, ethanol combination mixture of one of them, namely BCG, dinitrochlorobenze （DNCB）, realgar and phosphate buffer solution （PBS） on B16 transplanted murine tumor. Methods 30 cases of C57BL/6 mice were inoculated with 1 × 10^5 B16 under forward axillary skin, and were randomized into 5 groups, ethanol group, BCG ethanol group, DNCB ethanol group, realgar ethanol group, and PBS group. All in 0.1ml were injected into tumor twice a week every mouse, BCG ethanol group was injected with suspension mixture of 100% ethanol and 15μg of BCG, realgar ethanol group with suspension mixture of 100% ethanol and 1 mg realgar, DNCB ethanol group with solution of 100% ethanol and 1 mg DNCB, and PBS group with 0. 1ml of PBS. Results 1 case of mice in DNCB ethanol group died after 20 hours of the 3rd injection. 30 days later, all tumors of rest 29 mice were separated and weighed respectively, the weights of ethanol group, BCG ethanol group, DNCB ethanol group, realgar ethanol group, and PBS group were （2.19±1.69）, （1.76±1.32）, （0.13±0.10）, （2.61±1.45） and （3.97±1.01） gram every mouse respectively. There were statistically difference between DNCB ethanol group and any other group （P〈0.05）. Conclusions Intra-tumor injection of solution of ethanol with small dose of DNCB （lmg） may obviously inhibit B 15 cell growth, their inhibition ratio on tumor to PBS and purified ethanol are 96.73% and 94.06% respectively, and the solution might develop into a potential immuno-therapeutic local drugs.
出处
《世界肿瘤杂志》
2006年第2期86-88,94,共4页
Tumour Journal of the World
基金
华东医院2005年科研基金资助,致谢：复旦大学生命科学院黄伟达教授、沈水源博士的帮助及英文审定.
