摘要
以一个与甘蓝显性核不育相关的差异表达片段序列为信息探针,在NCBI与TAIR网站数据库中进行同源EST序列搜索,经人工拼接、RT-PCR克隆与序列分析验证,获得了青花菜快速碱化因子RALF(Rapid Alkalinization Factors)基因的cDNA全长序列,命名为BoRALFL1(GenBank序列登录号DQ059310)。该cDNA全长240bp,编码79个氨基酸,与电子克隆获得的序列完全相同。序列分析表明,编码蛋白存在前导信号肽与多个磷酸化位点,与同源基因RALFL8核酸序列在88bp上有82%的一致性,推导的氨基酸序列在74个氨基酸上存在56%的一致性,不同植物间氨基酸序列N-端差异大,C-端具有较高的保守性。
A differential TDF （Transcript Derived Fragments） in a cDNA-AFLP analysis of buds from male sterile and fertile lines of cabbage was used as a querying probe to blast the GenBank （ http： // www. ncbi. nlm. nih. gov/BLAST） and Arabidopsis （http： //www. arabidopsis, org/BIAST） database. Based on the assembled homologous cDNA sequences, a 490 bp cDNA was amplified and cloned by RT - PCR. Homologous analysis shows that the amplified cDNA has 82% identity on the nucleotide acid sequence, and 56% identity on the amino acids peptide with Arabidopsis gene RALFL$. We designated the gene as BoRALFL1 （GenBank accession number DQ059310）. We predicted a 240 bp ORF in BoRALFL1 and a 79 amino acids peptide was coded by this cDNA. Further analysis shows that the pepetide is possibly a preprotein with a signal pepetide and multi-phosphorylation sites, and the C-terminal amino acids of the pepetide are highly conserved among different plant species.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第3期561-565,共5页
Acta Horticulturae Sinica
基金
国家‘863’计划资助项目(2003AA207120)
国家自然科学基金资助项目(30370981
30471188
30400298)
