摘要
目的:探讨大鼠睾丸扭转复位后,附睾上皮细胞凋亡与肉碱分泌的关系。方法:24只健康成年雄性SD大鼠随机均分为:对照(扭转0 h)、A组(扭转2 h)和B组(扭转5 h),建立左侧睾丸扭转复位模型。TUNEL法检测附睾上皮细胞凋亡,DTNB法检测扭转侧附睾肉碱的含量。结果:睾丸扭转2 h复位后24 h,扭转侧附睾上皮细胞凋亡指数与对照组相比,上升不明显(P>0.05),肉碱含量改变不明显(P>0.05);扭转5 h复位后24 h,扭转侧附睾上皮细胞凋亡指数与对照组相比,上升极显著(P<0.01),肉碱含量明显下降(P<0.05)。结论:睾丸扭转2 h复位后24 h,附睾上皮细胞凋亡增加无显著性,其浓缩分泌肉碱的功能不受影响;扭转5 h复位后24 h,附睾上皮细胞凋亡严重,其浓缩分泌肉碱的功能下降。
Objective： To investigate the relationship between the apoptosis of epididymal epithelium and the change of epididymal carnitine following testicular torsion/detorsion in rats. Methods： Twenty-four healthy adult male Spragne-Dawley rats were randomly divided into 3 groups： Group A （2-hr torsion ）, Group B（5-hr torsion） and a control group（0-hr torsion）. The ipsilateral epididymides were collected for detecting the content of carnitine by DTNB technique and the apoptosis of epididymal epithelium by TUNEL technique. Results：Twenty-four hours after the treatment, there was no statistically significant difference in the apoptosis of epididymal epithelium and the content of epididymal carnitine between the 2-hr torsion/detorsion group and the control （P 〉 0.05 ）. However, there was statistically significant difference in the apoptosis of epididymal epithelium and the content of epididymal carnitine between the 5-hr group and the control （P 〈 0.05）. Conclusion ： Twenty-four hours after 2-hr testicular torsion/detorsion, the carnitine-concentrating function of the epididymis may remain normal and the apoptosis index of epididymal epithelium does not increase significantly, while one day after 5-hr testicular torsion/detorsion, the apoptosis index increases and the carnitine-concentrating function decreases.
出处
《中华男科学杂志》
CAS
CSCD
2006年第7期636-638,共3页
National Journal of Andrology
关键词
睾丸扭转
细胞凋亡
肉碱
附睾上皮细胞
大鼠
testicular torsion
apoptosis
camitine
epididymal epithelium
rat
