摘要
目的:评价隐形顺铂聚乳酸纳米微粒(CDDP-PLA-PEG-NP)联合nm23-H1基因对口腔癌靶向治疗的疗效。方法:用DMBA诱导建立金黄地鼠口腔颊癌模型,30只颊癌金黄地鼠随机分为3组,每组10只:①实验组:PEG-CDDP-PLA-NP静脉注射+脂质体包裹的Adeasy-nm23-H1质粒瘤内注射;②对照组Ⅰ:单行脂质体包裹的Adeasy-nm23-H1质粒瘤内注射;③对照组Ⅱ:单行PEG-CDDP-PLA-NP静脉注射。比较3组癌细胞的凋亡情况。结果:实验组、对照组Ⅰ和对照组Ⅱ完成治疗后癌细胞凋亡指数(AI)分别为31.46±2.371、0.04±1.422、2.63±1.96。实验组癌细胞凋亡指数显著高于对照组Ⅰ和对照组Ⅱ(P<0.05)。结论:隐形顺铂聚乳酸纳米微粒联合nm23-H1基因较单一nm23-H1基因治疗或隐形纳米靶向治疗显著地提高了对口腔癌的治疗效果,该方法有较大的发展前景。
Objective： To investigate therapeutic effect of Cisplatin loaded polylactic acid- polyethylene glycol nanoparticles（CDDP-PLA-PEG-NP） and nm23-H1 gene combinedtargeted therapy for oral carcinoma.Method：In DMBA-induces hamstersm oral carcinoma, 30 model hamsters were divided randomly into three groups far 10 animals in each group： ①ⅠGroup was treated with CDDP-PLA-PEG-NP plus nm23-H1 gene; ②ⅡGroup was treated with nm23-H1 gene alone;③ Ⅲ Group was treated with CDDP-PLA-PEG-NP alone. Apoptosis in tumor cells was compared among three groups.Result：Apoptoti index of Ⅰ-Ⅲ groups were 31.46±2.37,10.04±1.42 and 22. 63 ± 1.96 respectively; Apoptotic index of Ⅰ group was significantly higher than that of Ⅱ group and Ⅲ Group（ P 〈 0.05）. Conclusion： In comparison with nm23-H1 gene treatmeat alone or CDDP-PLA-PEG-NP treatmeat alone, CDDP-PLA-PEG-NP plus nm23-H1 gere can significantly increase therapeutic effect far oral carcinoma, this kind of treatmeat model is worth further exploring.
出处
《临床口腔医学杂志》
2006年第7期392-394,共3页
Journal of Clinical Stomatology
基金
重庆市科委应用基础研究项目(2003-43)
重庆市卫生局科研项目(2001年)
重庆医科大学科技基金(XB200207)
关键词
隐形纳米/长循环纳米
NM23-H1基因
顺铂
肿瘤
口腔
stealth nanoparticles/Long-circulating nanoparticles
nm23-H1 gene
cisplatin
neoplasm
oral cavity
