摘要
寡核苷酸基因指纹探针ＬＺＦＩ对大山雀青藏亚种（Ｐａｒｕｓｍａｊｏｒｔｉｂｅｔａｎｕｓ）和华北亚种（Ｐ．ｍ．ａｒｔａｔｕｓ）随机个体进行了基因指纹图的比较检测，结果如下：（１）Ｐ．ｍ．ｔｉｂｅｔａｎｕｓ个体在２．３ｋｂ处均有一条杂交谱带，而Ｐ．ｍ．ａｒｔａｔｕｓ个体的共有谱带则位于４．８ｋｂ处。不同的共有等位基因，是区别两亚种的遗传标记之一。（２）Ｐ．ｍ．ｔｉｂｅｔａｎｕｓ和Ｐ．ｍ．ａｒｔａｔｕｓ随机个体的平均谱带数分别是１４．６５条和１９．３０条；个体间基因指纹图的相似系数分别是０．１８和０．１３；Ｐ．ｍ．ｔｉｂｅｔａｎｕｓ和Ｐ．ｍ．ａｒｔａｔｕｓ个体之间基因指纹图的相似系数是０．０９。结果表明：Ｐ．ｍ．ａｒｔａｔｕｓ群体内的遗传变异性高于Ｐ．ｍ．ｔｉｂｅｔａｎｕｓ群体，且两亚种之间的变异性高于两亚种内部的变异性。（３）ＬＺＦＩ探针在Ｐ．ｍ．ｔｉｂｅｔａｎｕｓ随机个体检测中的鉴别几率为２．０４×１０－１２，在Ｐ．ｍ．ａｒｔａｔｕｓ则高达９．８×１０－１７。表明两亚种的基因指纹图均具有个体特异性，且Ｐ．ｍ．ａｒｔａｔｕｓ群体基因组的多态性较Ｐ．ｍ．ｔｉｂｅｔａｎｕｓ强。该结果为开展对大山雀种内分化的基因多?
comparative study was conducted to determine the DNA ‘fingerprints’ of P.m.tibetanus and P.m.artatus by employing the oligonucleotide probe LZF I technique.The important findings are as follows: (1) Identical bands were detected at the 2.3 kb in all of the randomly selected individuals of P.m.tibetanus,and at the 4.8 kb of the P.m.artatus samples.This difference in the alleles could be one of the important genetic markers used to classify the two subspecies. (2) The average bands were 14.65 in P.m.tibetanus, and 19.30 in P.m.artatus.The similarity coefficients among the individuals of P.m.tibetanus and P.m.artatsu were 0.18 and 0.13,respectively.The similarity coefficient between the subspecies was 0.09.These results indicated that the individuals of P.m.artatus had greater genetic variation than those of P.m.tibetanus,and the variations between the two subspecies were greater than between the individuals of each subspecies. (3) The probability of the LZF I probe in identifying the genetic variation among the individuals of P.m.tibetanus was 2.04×10 -12 ,while that was 9.8× -17 among the individuals of P.m.artatus,suggesting that there existed specificity of fingerprints among all the individuals of the two subspecies,and that the polymorphism of the genomic DNA in P.m.artatus was greater than that in P.m.tibetanus. The results reported in this paper are of importance in studying the genetic diversity and systematic position of the two species.
出处
《生物多样性》
CAS
CSCD
1996年第4期207-210,共4页
Biodiversity Science
关键词
大山雀
基因指纹图
遗传多样性
P. m. tibetanus,Parus major artatus, DAN fingerprint,genetic diversity
