摘要
目的探讨氟中毒大鼠的骨转换调控过程,分析转化生长因子-β(transforming growth factor-β, TGF-β)超家族成员骨形态发生蛋白(BMP)在氟骨症骨转换过程中的作用。方法4周龄雄性Wistar大鼠随机分为对照组和氟化钠组,氟化钠组饮用含氟(F-)150 mg／L的水溶液,对照组饮用自来水。每4周处死一批动物,共计6次,采用RT-PCR方法、免疫组织化学方法以及Western blot等方法,分析氟中毒大鼠长骨组织TGF-β超家族成员BMP-2、BMP-7、TGF-β1 mRNA及蛋白的表达情况。结果氟中毒大鼠骨组织中BMP-2、BMP-7、TGF-β1mRNA表达持续高于对照组,蛋白主要表达在骺板软骨增生期和肥大期细胞、关节软骨细胞、成骨细胞以及韧带细胞等部位。
Objective To analyze the effects of members of transforming growth factor-β （TGF-β） superfamily on bone turnover in fluorosis and the regulatory process. Methods Male wistar rats were randomly divided into two groups, i.e. sodium fluoride group and normal control group. Sodium fluoride group was given sodium fluoride（F^-） in drinking water containing 150 mg/L, and normal control group was given tap water only. The animals were bred for six months. Some of the rats were killed in each month. The expression of bone morphogenetic protein-2 （BMP-2）, BMP-7, TGF-β1 mRNA and protein were analyzed by RT-PCR, immunohistochemistry and Western blot. Results The expression of BMP-2, BMP-7 and TGF-β1 mRNA remained at high level in rats treated with sodium fluoride and these proteins mainly appeared in hyperplastic zone and hypertrophic zone of epiphyseal plate cartilages, articular cartilage cells, osteoblasts and ligament cells. Conclusions TGF-β superfamily members are involved in bone turnover in fluorosis via control osteogenesis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第4期374-378,共5页
Chinese Jouranl of Endemiology
基金
国家自然科学基金资助项目(30371251)
关键词
氟骨症
转化生长因子-Β
骨形态发生蛋白
骨转换
Skeletal fluorosis
Transforming growth factor-β
Bone morphogenetic proteins
Bone turnover
