摘要
目的研究白藜芦醇对人肝癌细胞HepG2的生长抑制作用以及对细胞间隙连接通讯(GJIC)的影响。方法利用MTT法观察白藜芦醇对HepG2细胞的生长抑制作用,流式细胞仪检测细胞周期的时相分布,使用荧光探针5'-CFDA/AM标记细胞,采用荧光漂白后重恢复技术(FRAP)和共聚焦显微镜技术观察白藜芦醇对HepG2细胞间隙连接通讯的影响。结果不同浓度的白藜芦醇处理细胞不同时间后,HepG2细胞的生长增殖明显受到抑制,抑制率最高达92.1%,各处理组间比较均有显著性差异(F=18.532,P<0.05);细胞周期分析显示,白藜芦醇能诱导HepG2细胞在S期停滞,抑制细胞DNA的合成并可诱导细胞凋亡;另外,白藜芦醇有恢复HepG2细胞间隙连接通讯的功能,且随浓度增加而增加。结论白藜芦醇可抑制HepG2细胞增殖,使细胞停滞于S期,并能诱导细胞凋亡;白藜芦醇还有恢复HepG2细胞间隙连接通讯功能的作用,提示此作用可能是白藜芦醇抑制HepG2细胞增殖和抗肿瘤作用的机制之一。
Objective To investigate the effects of the resveratrol on proliferation and gap-junctional intercellular communication （GYIC） in human liver cancer cell line HepG2. Methods MTT assay was used to observethe effects of resveratrol on HepG2 cell growth, and the distribution of cell cycles was detected with flow cytometry （FCM）. The effects of resveratrol on GJIC of HepG2 cells labeled with 5＇-CFDA/AM was examined with fluorescence redistribution after photobleaching （FRAP） and confocal microscope. Results The results of MTT assay indicated that the proliferation of HepG2 cells was significantly inhibited by resveratrol in a time-and dose-dependent manner. Resveratrol could arrest HepG2 cell growth in S phase, inhibit DNA synthesis and induce cell apoptosis. Furthermore, the levels of GJIC increased sharply after resveratrol treatment of the cells. Conclusion Resveratrol is capable of inhibiting HepG2 cell proliferation, causing cell growth arrest at S phase and inducing cell apoptosis. Increased GJIC level contributes to the effect of resveratrol in HepG2 cell proliferation inhibition and its cancer chemonreventive activity.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第7期963-966,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30300455)~~
关键词
白藜芦醇
细胞凋亡
HEPG2
细胞间通讯
resveratrol
apoptosis
HepG2
gap-junctional intercellular communication
