摘要
目的:研究胃癌中DNA倍体及其TIMP-2和E-cadherin的表达,探索胃癌侵袭转移的分子基础和可能机制。方法:采用免疫组化技术检测E-cadherin、TIMP-2在99例胃癌,16例癌周正常黏膜,16例胃癌远处转移和25例胃癌转移阳性的淋巴结中表达情况;选取其中47例胃癌,6例癌周正常黏膜及4例胃癌远处转移标本采用流式细胞术检测DNA倍体及S期分数。结果:TIMP-2表达与Borrmann’s分型、淋巴结转移和浸润深度有关;E-cadherin表达与肿瘤细胞分化、Lauren’s分型、Borrmann’s分型、淋巴结转移和浸润深度有关。DNA异倍体率与分化和淋巴结转移有关,S期分数(SPF)与肿瘤大小、分化及淋巴结转移有关。而且在癌与癌周非癌黏膜之间E-cadherin表达、DNA异倍体率和S期分数的差别具有统计学意义;TIMP-2与E-cadherin之间无相关性;E-cadherin表达与DNA倍体及S期分数呈正相关。结论:随着肿瘤的演进和异质化,TIMP-2和E-cadherin的异常表达及DNA异倍体和高S期分数也相应逐渐增加,提示它们在胃癌演进过程中起着关键作用,可以作为胃癌生物学行为的客观标志物。而且,这几种因素间的相互作用更加速了肿瘤演进过程。
AIM ： To investigate DNA ploidy and the expression of TIMP - 2 and E - cadherin in gastric carcinoma in order to understand its molecular basis and probable mechanism of invasion and metastasis. METHODS： Immunohistochemical methods were used to detect the expression for TIMP - 2 and E - cadherin in 99 cases of gastric carcinoma, 16 cases of adjacent noncancerous mucosa, 16 cases of distant metastases and 25 cases of metastatic lymph nodes. Flow cytometry DNA ploidy and S -phase fraction （SPF） analysis was performed on 47 cases of gastric cancer, 6 cases of adjacent noncancerous mucosa and 4 cases of distant metastasis cancer with the use of formalin - fixed paraffin embedded specimens. RESULTS ： The expression of TIMP - 2 was significantly correlated with Bonmann＇ s classification, LN metastasis and the depth of invasion. The expression of E - cadherin was significantly correlated with tumor cell differentistion, Lauren＇ s classification, Borrmann＇ s classification, LN metastasis and the depth of invasion. There was a positive relationship between DNA aneuploid rate and differentiation and LN metastasis. There was a positive relationship between SPF that is higher than 15% and tumor size, differentiation and LN metastasis. And there was a significantly difference between carcinoma and noncarcinoma when the expression of E - cadherin, DNA aneuploid rate and SPF were analyzed. There was no correlation between TIMP - 2 and E - cadherin. There was a positive relationship between DNA ploidy or SPF and the expression of E - cadherin. CONCLUSION ： As the development of tumor progression and heterogeneity, the abnormal expression of TIMP - 2 or E - cadherin or the rate of DNA aneupoid or higher SPF gradually correspondingly increases, suggesting that they play a crucial role in gastric carcinoma progression. Furthermore, each factor influences one another and further accelerates the process of tumor progression.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第7期1353-1358,共6页
Chinese Journal of Pathophysiology
基金
山东省教育厅课题资助(No.03K02)
