摘要
以大岩桐新生嫩叶为外植体进行了植株再生和快速繁殖研究。结果表明:诱导不定芽以M S+3%蔗糖+0.5%琼脂+0.05 m g/L NAA+0.50 m g/L 6-BA效果最好,分化率达100%;增殖培养以M S+3%蔗糖+0.5%琼脂+0.10 m g/L 6-BA+0.10 m g/L NAA培养基增殖倍数最大,为6.5倍;诱导不定根,在1/2M S+1.5%蔗糖+0.5%琼脂+0.10 m g/L NAA培养基上生根率为100%,达到植株再生和快繁的目的。
The plant regeneration and micropropagation of Sinningia speciosa young shoots as explants was studied in this paper. The results showed that the most suitable iduction culture medium is MS＋3% cane sugar ＋ 0.5 agar ＋ 0.05 mg/L NAA＋0.50 mg/L 6-BA, differentiation rate could reach 100%; the largest times of enrichment culture is 6.5 which is culture medium by MS＋3% cane sugar＋0.5% agar＋0.10 mg/L 6-BA＋0.10 mg/L NAA. The ideal culture medium for rooting is 2/1MS＋1.5% cane sugar＋0.5% agar＋0.10 mg/LNAA of with 100% rooting rate which is reach to plant regeneration and micropropagation.
出处
《甘肃农业科技》
2006年第6期10-11,共2页
Gansu Agricultural Science and Technology
关键词
大岩桐
组织培养
快速繁殖
Sinningia speciosa
Tissue culture
Mieropropagation
