摘要
目的探讨原发性高血压(EH)肾损害及肾实质性高血压胰岛素受体(IN SR)基因第17外显子多态性,从遗传学角度寻求其病因。方法应用多聚酶链反应和单链构象多态性分析(SSCP)等技术,对原发性高血压肾功能正常(EH-NRF组)、高血压肾损害肾功能衰竭(EH-CRF组)、肾实质性高血压肾功能正常(RH-NRF组)、肾实质性高血压肾功能衰竭(RH-CRF组)及正常血压(NC组)的全血IN SR基因第17外显子多态性进行研究。结果四组患者与正常对照组IN SR基因第17外显子SSCP均显示三种不同的带型,分别称为带型Ⅰ(野生型)、带型Ⅱ(突变型)、带型Ⅲ(突变型)。EH-NRF组及EH-CRF组突变频率(带型Ⅱ+带型Ⅲ)明显高于RH-NR组、RH-CRF组及NC组(P<0.05)。各带型在RH-NRF组、RH-CRF组及NC组间出现的频率无显著性差异。结论①IN SR基因第17外显子多态性与中国人EH有关,EH患者突变率明显高于RH患者。IN SR基因第17外显子多态性改变与EH是否并发肾损害无关。②肾实质性高血压患者无论是否并发肾功能衰竭,均未发现IN SR基因第17外显子多态性改变。
[Objective] To investigate the relationship between polymorphism of insulin receptor （INSR） gene Exon 17 with hypertensive nephrosclerosis and renal hypertension. [Methods] Polymerase chain relation （PCR） combined with single strand conformation polymorphism （SSCP） was used to detect and analyse the NsiI polymorphism of INSR gene Exon 17 in normal renal function of essential hypertension （EH-NRF）, renal failure of hypertensive nephroselerosis （EH-CRF）, normal renal function of renal hypertension （RH-NRF）, renal failure of renal hypertension （RH-CRF） and normal control subjects （NC）. [Results] Three different patterns of the PCR products in SSCP, which called band pattern Ⅰ , Ⅱ respectively, were presented in all four patient groups and normal control subject. The mutation frequency of EH-NRF and EH-CRF was remarkblely higher than those of RH-NRF, RH-CRF and NC （P〈0. 05）. there was no diference between patients of EH-NRF and EH-CRF. Among the patients of RH-NRF,RH-CRF and NC, there was no diference in frequency of all band pattern. [Conclusions] ①The polymorphism of INSR gene Exon 17 was associated with EH and the mutation frequency of EH was remarkblety higher （band Ⅱ ＋ Ⅲ ） than that of RIt. ②The polymorphism of INSR gene Exon 17 has unconcern with EH whether complicated by renal damage and with renal damage complicated by hypertension as well.
出处
《山东医药》
CAS
北大核心
2006年第19期12-14,共3页
Shandong Medical Journal
基金
青岛市科技计划项目(2001KNS-2E-48.2)
山东省医学会三等奖项目(2005)
青岛市科技进步二等奖项目(2005)
