摘要
目的 建立一种高效液相色谱法同时测定乌鸡白凤丸（浓缩丸）中丹参素及芍药苷的含量.方法 采用反相液相色谱法,以BDSC18柱（250mm×4.6mm,5μm）,为色谱柱：流动相为：A：0.3%乙腈磷酸溶液：0.3%甲醇磷酸溶液（1：1）,B：0.3%磷酸水溶液梯度洗脱;流速1.0mL·min^-1;检测波长230nm.结果 丹参素在0.104～1.04μg范围内线性关系良好（r=0.9999）,平均回收率为101.77%,RSD=1.31%;芍药苷在0.204～2.04μg范围内线性关系良好（r=0.9984）,平均回收率97.60%,RSD=0.65%.结论 该方法简便、准确,可作为该制剂的含量测定方法.
OBJECTIVE To establish the method for determining simultaneously the contents of Danshensu and Paeoniflorin in Wujibaifengwan（Concentrated Pills）by HPLC. METHODS Applying RP-HPLC with the analytical column BDSC18 （250mm×4. 6mm, 5μm）the mobile phase was composed of A： 0. 3%Acetonitrile phosphoric acid-0. 3 %methanol phosphoric acid （1：1）,B：0. 3 %phosphoric acid for the steps degree washes to take off,the flow rate was 1.0mL· min^-1,the detection wavelength was at 230nm. RESULTS The range of fine linear relation was 0. 104 ～ 1.04μg （r = 0. 9999） for Danshensu, 0. 204 ～ 2.04μg （r = 0. 9984） for Paeoniflorin. The average recoveries of Danshensu and Paeoniflorin were 101.77% （RSD= 1.31% ）,and 97.60% （RSD = 0.65%）.CONCLUSION The method is simple accurate and can be applied to the contens determination in this preparation.
出处
《海峡药学》
2006年第3期59-60,共2页
Strait Pharmaceutical Journal
