摘要
目的探讨抗血管内皮生长因子(VEGF)发夹状核酶基因对白血病细胞裸鼠体内生长和肿瘤内血管生成的影响。方法采用脂质体介导的方法将抗 VEGF 发夹状核酶基因真核表达载体pcDNA-RZ 转染白血病细胞系 K562,G418抗性筛选获得阳性克隆;抽提基因组 DNA,用 PCR 方法验证核酶基因已转入 K562细胞;荧光定量 PCR 和免疫印迹反应检测白血病细胞中 VEGF mRNA 和蛋白表达量的改变;将白血病细胞皮下接种 BALB/c 裸鼠,观察转染细胞在裸鼠体内成瘤及生长情况;组织形态学和免疫组织化学法检测裸鼠移植瘤微血管密度(MVD)。结果抗 VEGF 发夹状核酶基因真核表达载体 pcDNA-RZ 转入白血病细胞系 K562(K562/RZ),G418筛选两周获得阳性克隆,PCR 检测证实核酶基因整合入白血病细胞基因组 DNA;与 K562及 K562/PC 细胞(转染空质粒的 K562细胞)相比,K562/RZ 细胞 VEGF mRNA 和蛋白的表达量明显降低。接种 K562、K562/PC 和 K562/RZ 细胞组小鼠肿瘤终体积分别为(3.21±0.89)cm^3,(3.42±1.01)cm^3,(1.71±0.94)cm^3;肿瘤重量分别为(4.43±0.87)g,(3.96±0.94)g,(2.24±0.56)g;瘤体内 MVD 分别为4.70±1.25,4.67±1.31和1.80±1.55。以上各组之间的差异均有统计学意义(P<0.01)。结论转导抗 VEGF 发夹状核酶基因能减少白血病细胞中 VEGF 的合成,细胞裸鼠致瘤能力明显减弱,瘤体内血管形成能力降低。
Objective To explore the effect of anti-VEGF hairpin ribozyme gene on the tumor cell growth and tumor angiogenesis in nude mice. Methods The recombinant eukaryotic expression plasmid pcDNA-RZ containing anti-VEGF hairpin ribozyme gene and the empty vector plasmid pcDNA were introduced separately into K562 cells by lipofectamine mediation and positive clones were screened by G418. Ribozyme gene in K562 cells was confirmed by PCR. Fluorescent real time RT-PCR and Western blot were used to detect the expression of VEGF mRNA and protein in the leukemia cells. The tumorigenicity of transfected K562 cells were transplanted in nude mice and tumor microvascular density （MVD） were observed by morphology and vWF immunohistochemistry stain. Results Stable expression of the ribozyme gene in K562 cells was confirmed by PCR. The level of VEGF mRNA and protein decreased dramatically in K562/RZ cells when compared with K562 or K562/PC（ K562 cells transfected with empty vector） cells. The tumor volumes were （4.43 ± 0.87 ）, （3.96 ± 0.94 ）, （2.24 ± 0.56 ） cm^3 ; tumor weight was （4.43 ± 0.87 ）, （ 3.96 ± 0.94）, （2.24 ±0.56）g;and tumor microvascular density was 4.70 ± 1.25, 4.67 ± 1.31,1.80 ± 1.55 in K562, K562/PC and K562/RZ cell groups, respectively. Conclusion Transfection with anti-VEGF ribozyme gene can inhibit tumor growth and vessel formation by down-regulating the VEGF gene expression in K562 cells.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2006年第7期465-469,共5页
Chinese Journal of Hematology
