摘要
目的探讨氧化应激在高氧肺损伤发生机制中的作用,并观察N乙酰半胱氨酸(N-ace-tylcysteine,NAC)的干预效果,以期为临床防治高氧肺损伤提供一条新的有效途径。方法光镜观察肺组织病理形态学;ELISA方法检测各亚组大鼠血浆8异前列腺素F2α含量,并比较高氧模型组与空气组及不同剂量NAC组大鼠血浆8异前列腺素F2α含量之间的差别。结果高氧模型组第3、7天,肺泡大小不等,肺泡内有出血和炎症细胞浸润;第14、21天肺泡间隔增厚。高氧+大剂量NAC组第3、7天肺泡内极少量红细胞渗出;第14、21天肺泡间隔无明显增厚。各时间点血浆8异前列腺素F2α含量高氧模型组分别为(28.33±5.57)pg/ml、(51.21±15.01)pg/ml、(84.54±14.85)pg/ml、(43.14±11.37)pg/ml,空气组为(19.09±5.57)pg/ml、(18.24±5.91)pg/ml、(17.00±5.58)pg/ml、(17.85±5.00)pg/ml,高氧+大剂量NAC组为(20.90±4.33)pg/ml、(37.30±12.86)pg/ml、(51.99±11.91)pg/ml、(29.37±9.41)pg/ml。高氧模型组各时间点血浆8异前列腺素F2α含量与空气组、高氧+大剂量NAC组比较差异均有统计学意义(P<0.05),与高氧+小剂量NAC组比较差异无统计学意义(P>0.05)。结论氧化应激与高氧肺损伤密切相关,NAC对高氧肺损伤的氧化应激作用具有明显的干预作用,其作用具有剂量依赖性。
Objective To explore the effects of oxidative stress in the pathogenesis of lung injury and observe the influence of N-acetylcysteine （NAC）. Methods The lung histopathology was observed by light microscope. The level of 8-iso-prostaglandin F2α1pha （8-iso-PGF2α） in blood plasm were measured by ELISA. The difference of 8-iso-PGF2α in blood plasm in air group, different dose NAC groups between hyperoxia-model and the air group was compared. Results In hyperoxia-model group, the inequality of size of lung alveoli, hemorrhage and inflammatory cell infiltration in lung alveoli were observed on the 3rd and 7th day. The alveolar septum was thick in the hyperoxic-damaged lungs on the 14th and the 21st day. In hyperoxia＋high-dose NAC group, very small amounts of red blood ceils leaked out into alveoli on the 3rd and 7th day and alveolar septum had no thickening obviously on the 14th day and the 21st day. The level of 8-iso-PGF2a in blood plasm in hyperoxia-model group [（28.33±5.57） pg/ml, （51.21±15.01） pg/ml, （84.54±14.85） pg/ml and （43.14±11.37） pg/ml at every time points] was higher than that of the air group and hyperoxia＋ high-dose NAC group（P〈0.05）. Conclusions Oxidative stress reaction is closely related to hyperoxia-induced lung injury. NAC has interventional effect on the pathological process of hyperoxia-induced lung injury and its protection effect was dose dependent.
出处
《中华围产医学杂志》
CAS
2006年第3期177-180,T0001,共5页
Chinese Journal of Perinatal Medicine
基金
暨南大学自然科学基金项目(620008)
广州医学院科学研究项目(2003-13)
