摘要
采用T4噬菌体表面展示系统,将CCK-33肽四多串联体及八多串联体展示在T4噬菌体表面,并研究其免疫原性。将CCK四串联体和八串联体与SOC基因缺失的、依赖溶菌酶的噬菌体ΦT4-Z1重组,获得重组噬菌体ΦT-4CCK和ΦT-8CCK。SDS-PAGE和Western Blot检测结果表明,CCK多串联体蛋白已经成功展示在T4噬菌体表面,其融合蛋白分子量约30 ku和41 ku,能够与CCK标准阳性血清发生特异性反应。以重组噬菌体为免疫原制备油佐剂疫苗主动免疫胡须肉鸡后,抗体浓度显著升高,肉鸡的体重、采食量都有所提高,而料肉比有所下降。结果说明,鸡CCK蛋白展示在T4噬菌体表面具有良好的免疫原性。
The 4 and 8 repeats of CCK-33 protein was displayed on phage T4 surface using phage T4 surface display system, and its immunogenicity also was studied. The 4 and 8 repeats of CCK- 33 gene were recombined with a SOC gene deleted lysozyme-dependent phage ФT4-Z1 to yield recombinant phage ФT-4CCK and ФT-8CCK. SDS-PAGE analysis result indicated that the recombinant CCK protein was displayed on the surface of phage T4 successfully and their molecular were about 30ku and 41ku, which further tested by Western-Blot demonstrating specific reaction with CCK antibody. The recombinant ФT-4CCK and ФT-8CCK were used as an antigen to prepare oilemulsion vaccines. After Hu-xu chicken were immunized with oilemulsion vaccines, the concentration of anti-serum, the body weight and feed intake of treated group were improved compared with the control group, but the feed meat ratio was decreased. The result showed that the recombinant CCK protein being displayed on phage T4 had good immunogencity.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第7期705-710,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
广东省自然科学基金团队项目(04205804)
