摘要
目的探讨内源性一氧化氮(NO)水平与慢性酒精性肝脂肪变性之间的关系。方法选取健康雄性清洁级SD大鼠32只,随机分成A、B、C、D4组,每组8只。A、B、C组分别采用酒精灌胃4、8、12周,制作慢性酒精性脂肪肝模型;对照组用相同剂量生理盐水灌胃12周。到期后采血分离血清测NO水平,并处死动物取肝脏,病理检查用半定量法进行肝脏脂肪变性评分。结果(1)A、B、C组肝脏脂肪变性评分分别为1.63±0.51、2.12±0.64和1.50±0.53,与对照组比较差异均具有统计学意义(P<0.05或0.01);(2)A、B、C组的血清NO水平分别为(76.35±10.56)μmol?L、(104.17±25.6)μmol?L和(54.30±7.41)μmol?L,对照组为(46.17±5.25)μmol?L,A、B组均较对照组明显升高(均P<0.01),而C组的水平已接近对照组(P>0.05);(3)4组的肝脏脂肪变性评分与血清NO水平之间存在正相关关系。结论大鼠在发生酒精性脂肪肝过程中,同时存在血清内源性NO含量的改变,酒精性肝细胞脂肪变性可能与内源性NO有关。
Objective To establish the animal model of alcoholic liver disease（ALD） and to evaluate the relation of nitric oxide with the severity of ALD. Methods Thirty two male Sprague-Dawley rats were randomly divided into four groups. Rats ingroup 1 （control group） were intragastrically infused with normal saline. The other three groups were intragastrieally infused with 56% （vol/vol） of ethanol for 4, 8 and 12 weeks respectively. At the end of 4, 8 and 12 weeks the blood samples were collected for nitric oxide measurement. Then the rats were sacrificed and liver specimens were obtained for histological examination. The degree of hepatic steatosis and activity of alcoholic hepatitis were evaluated by semi-quantitation method. Results The score of severity of steatosis were 1.63±0.51 （P〈0.05）, 2.12±0.64 （P〈0.01） 和 1.50±0.53 （P〈0.05） in 3 alcohol groups. The levels of nitric oxide in 3 alcohol groups were （76.35±10.56）μmol/L（P〈 0.01）, （104.17±25.59） μmol/L （P〈0.01） and （54.30±7.41）μmol/L （P〉0.05） ;that of control group was （46.17±5.25）μmol/L. Conclusion The results revealed that chronic alcohol infusion could lead to hepatic steatosis. Alcohol may contribute to significant increase of endogenous nitric oxide levels, which may be correlated with the severity of hepatic steatosis.
出处
《浙江医学》
CAS
2006年第6期439-441,共3页
Zhejiang Medical Journal
关键词
酒精
一氧化氮
酒精性肝病
Alcohol Nitric oxide Alcoholic liver disease
