摘要
目的:探讨功能矫形后退大鼠下颌后髁突软骨改建的分子调控机制。方法:SD大鼠40只,实验组配戴模拟临床功能矫治器,强制大鼠下颌后退,对照组不戴模拟矫治器,实验后3天、1、2、3周处死大鼠取髁突,HE染色观察组织学变化,免疫组织化学方法结合图像分析检测TGF-β1、TGF-βR1、IGF-1在髁突中的表达及分布。结果:实验组大鼠下颌后退1～2mm,镜下见颞下颌关节髁突软骨厚度增加,以生发层和过渡层增加明显,细胞数增多,细胞体积增大,核肥大深染。髁突各层软骨细胞均有TGF-β1、TGF-βR1和IGF-1的表达,免疫组织化学阳性染色相对面积和积分灰度的比较显示:在功能矫形后退下颌后,实验组TGF-β1、TGF-βR1和IGF-1的表达较对照组明显增强,其差异具有统计学意义(P<0.05)。结论:功能矫形后髁突软骨表现为增生改建、分化功能增强,其发生与TGF-β1、TGF-βR1和IGF-1的表达密切相关。
Objective： To investigate the molecule mechanism of growth rat condylar cartilage remodeling after functional retraction of mandible, neth .ods：Forty SD rats were randomly divided into experiment group and control group. The mimic functional appliances were used in experiment group to back off the mandible. The rats were killed for condylars after 3 days, 1 week, 2 weeks or 3 weeks. HE staining was used to detect the histological changes and immunohistochemical technique was used to detect the expression of TGF-β1,TGF-β R1 and IGF-I in condylar. Results：In experimental group,the rat mandibles fell back for 1-2 mm,and incrassation of condylar cartilage was detected in procreative layers and transitional layers. The amount and volume of the cells was aug- mented,and the karyon was increscent and fuscous, while the cytoplasm was reduced. TGF-β1,TGF-βR1 and IGF-1 were expressed throughout the condylar cartilage and the immunostain levels of experiment group were higher than those of control groups（P 〈 0.05）. Conclusion： The condylar cartilage remodeling and enhanced differentiation are related with the expression of TGF-β1 ,TGF- β R1 and IGF-1.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第8期702-705,F0003,共5页
Journal of Nanjing Medical University(Natural Sciences)
