摘要
目的:寻找并鉴定MUC4HLA-A0201限制性细胞毒性T细胞(CTL)表位。方法:结合2种常用表位预测数据库(SYFPEITHI和ProPred-I)和工具,应用多种参数和方法进行综合分析,包括MHC结合力、蛋白酶体切割位点等综合预测方法。预测得到的抗原表位合成相应抗原肽。用T2细胞株测定各肽与HLA-A0201分子的亲和力。结果:综合分析预测得到HLA-A0201的5个可能表位;合成抗原肽经纯化后纯度>95%;在5个候选表位肽中,LLLGVGTFV(1125-33)和LLGVGTFVV(1126-34)2个九肽与HLA-A0201的结合力较强。结论:多参数表位预测结果和结合力分析实验结果一致性较好,两者联合应用初步认为LLLGVGTFV(1125-33)和LLGVGTFVV(1126-34)2个九肽为MUC4HLA-A0201限制性CTL表位的可能性最大。
Objective： To predict and identify HLA-A^＊0201-restricted cytotoxic T lymphocyte epitope from MUC4. Methods： 2-epitope prediction databases （SYFPEITHI and ProPred-I） combined with several parameter and methods were used to analyzed. MHC binder and proteasome/immunoproteasome cutting site. T2 cell line was used to determine the peptide binding with HLA- A^＊0201 molecule. Results： After comprehensive analy.sis, 5 candidate HLA-A^＊0201 epitopes were acquired. Amorig five predicted epitopes, LLLGVGTFV（1125-33） and LLGVGTFVV（1126-34） were determined as the two with more HLA-A^＊0201 affinity. Conclusion： Our results suggest that multi-parameter prediction accorded with peptide binding assay. In this study, both epitope prediction and peptide binding assay show that LLLGVGTFV（1125-33） and LLGVGTFVV（1126-34） may be the HLA-A^＊0201-restricted epitope from MUC4.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第7期513-515,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助项目(30471691)
