摘要
利用噬菌体随机十二肽库对金属N i2+进行了结合肽筛选,经5轮生物淘洗、噬菌体扩增和DNA测序,获得8条多肽序列,分析发现其中富含组氨酸(4~6个/肽).结果表明,组氨酸的存在对蛋白质和金属N i2+的结合起着关键作用.
Phage random 12-peptide library was used for selecting peptide binding for Ni^2＋ . After five rounds of biopanning, phage amplification and DNA sequencing, a group of 12 - peptides were obtained from the library. The sequences of these DNA coding peptides were sequenced and identified, containing four to six histidine residues. The results showed histidine residues were very important in the binding peptides for Ni^2＋. Fig 1, Tab 1, Ref 11
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2006年第3期379-380,共2页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金资助项目(No.30270041)
西南交通大学引进人才科研启动基金支持项目~~
关键词
NI^2+
噬菌体随机肽库
结合肽
筛选
Ni^2＋
phage random peptide library
binding peptide
biopanning
