摘要
通过肾癌细胞的体外实验，应用MTT方法，观察了化疗致敏剂搏定对细胞毒药物长春新碱和阿霉素抗RCC－925肾癌细胞效应的体外增效作用．结果发现：RCC－925肾癌细胞系对长春新碱高度耐药，对阿霉素低度耐药，半数致死浓度分别为14726．4μg／ml和1．559μg／ml。异搏定1.25μg／ml即可显著提高长春新碱和阿霉索的细胞毒性，增效幅度随异搏定浓度的升高而增强，增效倍数对长春新碱为1．3×104～1．5×105倍，对阿霉素为3．5～25．1倍。提示异搏定对RCC－925肾癌细胞的多重耐药性具有显著的逆转作用．
Using MTT method, the effects of verapamil on the enhancement of cytotoxicity of Vincristine(VCR)and Adriamycin (ADM) against renal cell carcinoma cell line RCC-925 in vitro were investigated. RCC-925cell line showed high resistance to VCR and moderate resistance to ADM. The LC50. was 14726. 4μg/ml for VCRand 1. 559μg/ml for ADM,suggesting that RCC-925 cell line have the natural multidrug resistance. Verapamil atthe concertration above 1. 25μg/ml could significantly enhance the cytotoxicity of VCR and ADM,the extent of enhancement was correspondingly increased as the dose was increased and the multiplication was from1. 3 × 104 to 1. 5 × 105 in VCR and 3. 7 to 25. 1 in ADM. The results suggested that verapamil dramatically reversemultidrug resistance in RCC-925 cells.
出处
《江苏医药》
CAS
CSCD
1996年第6期375-377,共3页
Jiangsu Medical Journal
关键词
多重耐药
肾肿瘤
异搏定
Multidrug resistance Kidney neoplasms Carcinoma Verapamil
