摘要
目的:探讨maspin及CD44V6在非小细胞肺癌(NSCLC)组织中的表达及临床意义,分析maspin与CD44V6的表达有无相关。方法:应用免疫组织化学PictureTM通用型二步法检测94例NSCLC组织、54例正常支气管粘膜组织、28例肺良性病变中maspin和CD44V6的表达情况。结果:maspin在正常支气管粘膜组织及肺良性病变中的阳性表达率分别为100%和89.28%,在肺癌组织中的阳性表达率为54.26%,正常支气管粘膜组织与肺癌之间、肺良性病变与肺癌之间的阳性表达率差异有显著意义(P<0.05),maspin阳性表达率与NSCLC临床分期、淋巴结转移成负相关,与病理类型、组织分化程度无关。CD44V6在NSCLC中的阳性表达率68.09%,与临床分期、淋巴结转移成正相关,与病理类型、组织分化程度无关;而在正常支气管粘膜组织及肺良性病变中无阳性表达。maspin基因表达与CD44V6呈负相关(r=-0.213,P<0.01)。结论:maspin和CD44V6在肺癌的发生、发展、转移过程中发挥调控作用,可作为判断肺癌预后和转移的重要生物学指标。
Objective： To study the expression of maspin and CD44V6 in human non-small cell lung cancer. Methods： Picture^TM, an immunohistochemical method, was used to detect CD44V6 and maspin protein expression in 94 lung cancer samples, 54 normal bronchial mucosas and 28 benign tumor samples. Results： The positive rate of maspin was 100%, 89.28% and 54.26% in normal bronchial epithelial cells, benign tumors and non-small Cell lung cancer, respectively （P〈0.05）. The expression of CD44V6 was negative in normal bronchial mucosa and in benign tumors, and 68.18% in non-small cell lung cancer. The expression of maspin and CD44V6 correlated with lymph node metastasis and TNM staging, but not with histological type and classification degree. The expression of maspin was negatively correlated with CD44V6 （r=-0.213, P〈0.01）. Conclusion： Maspin and CD44V6 are involved in carcinogenesis. The levels of maspin and CD44V6 may be a sensitive predictor of metastasis and prognosis in NSCLC.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2006年第12期712-715,共4页
Chinese Journal of Clinical Oncology
