摘要
实验对一株能高效降解几种有机磷农药菌株JSO18的降解酶进行分离和纯化。经分析该降解酶主要位于细胞间质，最适盐析硫酸铵饱和度为60％～70％，粗酶液经硫酸铵沉淀、Sephade。G-100凝胶过滤和DEAE-52柱层析得到较高纯度的酶液。通过PAGE和SDS—PAGE电泳测定该酶分子量约为37KDa．N末端16个氨基酸残基测序的结果为：GAPFQNDVPPACYRFR。
The organophosphorus pesticide degrading enzyme produced from strain JSO18 was purified by ammonium sulfate precipitation, Sephadex G - 100 gel filtration and chromatography on DEAE - 100 . This clegrading enzyme was mainly in the periplasmic space . The test results showed that the optimal concentration of ammonium sulfate was about 60%. The molecular weight of the enzyme was about 31KDa by PAGE and SDS - PAGE.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2006年第3期462-466,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
农业部948项目(2003-T17)
关键词
有机磷农药降解酶
电泳
层析
纯化
测序
organophosphorus pesticide degrading enzyme
electrophoresis
chromatography
purification
sequence
