摘要
目的利用人类性染色体Amelogenin同源基因在X、Y染色体上序列长度的差异,选择设计引物,对考古样本进行古DNA性别信息研究。方法采用苯酚/氯仿-二氧化硅-超滤离心方法提取东岭墓葬群殉人骨骼、牙齿古DNA,PCR扩增,非变性聚丙烯酰胺凝胶电泳(PAGE)分离和检测古DNA扩增片段。结果8个墓葬16个样品中有7个样品出现阳性扩增检测,目标基因片段清楚,男性为二条带(X、Y),女性为一条带(X),牙齿样品检测成功率优于骨骼样品。结论改进的苯酚/氯仿—二氧化硅—超滤分离法是较好的古DNA提取方法,具有降低PCR抑制剂、消耗成本低和提取成功率高等特点。基于人类X、Y染色体Amelogenin同源基因的古DNA性别分析方法可成为分子考古重要的技术方法。
Objective Based on the sequence differences of Amelogenin homologous gene in the X and Y chromosomes, a pair of specific primers was designed to identify the sex of archaeological samples. Methods Ancient DNA fragments were extracted from the bones and teeth of sacrificial slaves with an improved method that combines phenol-chloroform extraction, silicon dioxide adsorption with uhrafiltration concentration. The polyacrylamide gel electrophoresis （PAGE） was used to detect PCR products. Results Seven in sixteen samples from eight graves showed positive results and the targeted segments were visible： a male with two bands of 106bp （Amel-X） and 112 bp （Amel-Y）, while a female with only one band of 106bp （Amel-X）. Ancient DNA analyzing results from tooth samples are more marked than that from bones. Conclusion The improved extraction method is more effective for ancient DNA extraction, which reduced the PCR inhibitors and lowered experimental costs. The sex determination technology based on Amelogenin homologous gene is an important and feasible method in the molecular archaeological research.
出处
《法医学杂志》
CAS
CSCD
2006年第3期213-216,共4页
Journal of Forensic Medicine
基金
陕西省科学技术厅自然科学基金资助项目(2004D13)
