摘要
目的探索激光显微技术(lasercapturemicrodissectionsystem,LCM)捕获口腔上皮细胞,并进行STR-DNA分型检测的方法。方法用VERITAS显微切割仪红外低能激光显微捕获一定数量口腔上皮细胞,进行ProfilerPlus试剂盒STR复合扩增,检测DNA基因型。结果7~8个口腔上皮细胞能成功获得STR-DNA分型。3~4个口腔上皮细胞不能成功获得STR-DNA分型。结论激光显微捕获作为一种分离单个细胞的新技术,对于微量口腔上皮细胞的STR-DNA分型是可行的。
objective The STR genotypping of trace oral epithelial cells which are microdissected by laser capure microdissection system (LCM) is explored. Methods The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures. Results DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed. Conclusion It is viable in genotyping of trace oral epithelial cells by Laser Capure Microdissection as a new technology of seperating single cell.
出处
《法医学杂志》
CAS
CSCD
2006年第3期196-197,203,共3页
Journal of Forensic Medicine