摘要
目的 测定断血流中主要成分断血流皂苷A的含量。方法 采用反相高效液相色谱法。色谱柱DISCOVERY C18（4．6mmx250mm，5μm），流动相为甲醇-水（73：27），流速0．8ml／min，检测波长250nm。结果断血流皂苷A在15．0—39．0μg。ml^-1范围内呈良好的线性关系，平均加样回收率为98．5％，RSD为1．3％（n=9）。结论 本方法简便、快捷，重现性好。
Aim To establish a quantitative method for determination of dinodiside A in Clinopodium herb. Methods High performance liquid chromatography （HPLC） was employed for detemination. The sample, extracted with refluxing ethanol and columnied by macropore resin, was separated on DISCOVERY Cls Column（4.6ram × 250ram, 5μm）, with a mobile phase consisting of methanol and water（73：27）, a velocity of 0.8ml/min, a detecting wavelength at 250nm. Resuits Within the range of 15.0 - 39.0μg·ml^-1 of clinodiside A,the calibration curve was linear（ r = 0.999 8）.The mean recovery rate was 98.5 %, RSD = 1.3 % （ n = 9）. The content of clinodiside A in Clinopodium plants was 0.03 % - 0.07%. Conclusions The method was very stable, accurate, and can be used as a quality standard for Clinopodium herb.
出处
《解放军药学学报》
CAS
2006年第3期217-219,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
