摘要
目的探讨低氧预处理对大鼠晕船程度及对脑区与血清中铁含量的影响。方法大鼠分为3组:空白对照组(Ⅰ)、单纯晕船组(Ⅱ)、低氧+晕船组(Ⅲ)。Ⅲ组先接受模拟低氧处理4 h.d-1,共5 d。而后Ⅱ、Ⅲ组接受晕船刺激2 h.d-1,共5 d。记录高龄土摄入量,实验结束取大鼠大脑皮质、小脑、脑干、下丘脑及血清,原子吸收法测定样本铁含量。结果Ⅱ、Ⅲ组高龄土摄入量〔(0.927±0.219)g(、1.707±0.534)g〕均高于Ⅰ组〔(0.302±0.241)g(P<0.01)〕,且Ⅲ组高于Ⅱ组(P<0.01);Ⅱ、Ⅲ组大脑皮质、小脑、脑干及血清中铁含量均低于Ⅰ组(P<0.01),且Ⅲ组均低于Ⅱ组(P<0.01);Ⅱ、Ⅲ组下丘脑中铁含量接近Ⅰ组;高龄土摄入量与大脑皮质、小脑、脑干与血清中铁含量均呈负相关(r为-0.601^-0.541)(P<0.05),而与下丘脑中铁含量相关性无统计学意义。结论低氧预处理加重晕船程度,机制可能与晕船后多脑区中铁含量进一步降低有关,脑铁代谢在晕船过程中可能发挥重要作用。
Objective To investigate the effect of hypoxic preconditioning on severity of seasickness, and to detect iron concentrations in brain and serum of rat. Methods Rats were divided into 3 groups： control group（ Ⅰ ）, rota- tion group （ Ⅱ ）, hypoxic preconditioning ± rotation group （Ⅲ） Groupm were first exposed to simulated hypoxia for 4 h per day for 5 days. Then Group Ⅱ, Ⅲ were treated with simulated seasickness stimulation for 2 h per day for 5 days. The intake of kaolin were recorded each day. Animals were executed immediately after the last seasickness stimulation, and samples of cerebral cortex, cerebellmn, brain stem, hypothalamus, and serum were obtained to detect iron concentrations by atomic absorption spectrometry. Results Both Group I] and m took more kaolin[ （0.927 ± 0.219 ） g, （1. 707 ± 0.534） g] than Group Ⅰ [ （0.302 ± 0.241 ） g] （ P 〈 0.01 ）, and Group m took more kaolin than group Ⅱ（ P 〈 0.01 ）. The iron concentrations in cerebral cortex, cerebellum, brain - stem and serum of both Group Ⅱand Ⅲ were significantly lower than those of GroupⅠ （P〈0.01）, and those of Groupm were still lower than those of GroupⅡ （P〈0.01）, but no difference of iorn concentration in hypothalamus among the three groups was observed（ P 〉 0.05）. Negative correlations between kaolin intakes and iron concentrations in cerebral cortex, cerebellmn, brainstem, serum （r=-0.601～-0.541） （P 〈 0.05） but not in hypothalamus were observed. Conclusion Hypoxic preconditioning may aggravate the severity of seasickness in rats. It may relate to further decrease in iron concentrations of brain. So iron metabolism in brain may play an important role in the development of seasickness.
出处
《解放军预防医学杂志》
CAS
北大核心
2006年第3期168-170,共3页
Journal of Preventive Medicine of Chinese People's Liberation Army
关键词
低氧预处理
晕船
脑
血清
铁元素
hypoxic preconditioning
seasickness
brain
serum
iron
