摘要
本文研究了草酸和BTH(苯并噻二唑)溶液对黄瓜幼苗霜霉病的抗性诱导及病程相关蛋白的积累。结果表明:10mmol/L草酸或0.5 mmol/L BTH均能诱导黄瓜对霜霉病产生局部和系统抗性,且抗性的持久期均在15 d以上。BTH处理或接种霜霉菌都可系统诱导黄瓜叶片胞间隙液产生分子量分别为33、27和22 kD的蛋白,而草酸没有诱导这3种蛋白的表达。BTH或草酸处理均可引起POD活性的升高,但和对照相比并没有新的POD同工酶表达,POD活性升高与黄瓜对霜霉病的抗性有关。
The accumulation of pathogenesis-related proteins and disease resistance against downy mildew in cucumber plants were analyzed using BTH and oxalic acid. Local and systemic resistance against downy mildew were both rapidly induced in cucumber plants after treatment with 0.5 mmol/L BTH or 10 mmol/L oxalic acid, and duration of the induction could persist for 15 d. BTH or inoculation both systemically induced three proteins of 33, 27 and 22 kD in intercellular fluids, while oxalic acid did not induce the three proteins. BTH or oxalic acid treatment both induced the increase of POD activities, while no new POD isoenzymes were expressed in treated seedlings. The increase of POD activities was related to induced resistance of cucumber to downy mildew.
出处
《植物病理学报》
CAS
CSCD
北大核心
2006年第3期238-243,共6页
Acta Phytopathologica Sinica
基金
西北农林科技大学青年学术骨干支持计划项目
国家"十五"科技攻关重大专项课题(2004BA516A09)
西北农林科技大学科研专项基金(042M083)
关键词
黄瓜霜霉病
诱导抗性
BTH
草酸
病程相关蛋白
POD
cucumber downy mildew
induced resistance
BTH
oxalic acid
pathogenesis-related proteins
POD
