摘要
目的:探讨高压二氧化碳(CO2)对胃癌细胞腹腔侵袭转移能力的影响。方法:体外培养高侵袭力人胃癌MKN45细胞株,在高压CO2(25 mmHg)及不同持续时间(1、2、3 h)干预后,应用流式细胞技术,检测其MMP2和MMP9的表达水平变化。结果:高压CO2作用后胃癌MKN45细胞株MMP2和MMP9蛋白表达有所变化。与常规细胞培养比较,MMP2和MMP9表达差异较为明显(P<0.001,P<0.01)。结论:CO2对胃癌细胞的侵袭能力会产生一定的影响,高压CO2环境对胃癌细胞的侵袭能力影响较大。
Objective：To explore the effects of high-presure carbon dioxide on the abdominal metastatic capability of gastric carcinoma ceils line. Methods：The gastric carcinoma cell line M KN45 was cultured in vitro and treated with 95.5% carbon dioxide under height presure（25 mmHg ）for different periods of time（ 1,2,and 3 h）. And then MMP2 and MMP9 were measured by flow cytometry. Control groups were exposed to common culture without CO2. Results：After the treatment with carbon dioxide,the expression of MMP2 and MMP9 in MKN45 line were increased significantly （ P 〈 0. 001 ,P 〈 0.01 ） when compared with those in the control. Conclusion ： Carbon dioxide would produce in certain degree effect on metastatic capability of gastric cancer cells. Heigh- expression of carbon dioxide environment could influence remarkably the invasiveness of gastric cancer cells.
出处
《华北国防医药》
2006年第3期156-158,共3页
Medical Journal of Beijing Military Region
