摘要
目的:探讨共刺激分子B71和B72在大鼠肝脏热缺血伤再灌注损伤模型中的表达情况及其免疫学意义。方法:将雄性Wistar大鼠随机分为3组:A组(缺血30min再灌注24h)、B组(缺血60min再灌注24h)、C组(假手术组)。模型制备参考Ohmorid的方法。分别取模型之肝左叶,采用实时反转录聚合酶链反应(PTPCR)检测B71和B72在3组肝左叶组织中mRNA表达情况。结果:B71和B72mRNA在C组以极低水平表达,而在A组、B组表达却明显增多(P<0.01),且B组高于A组(P<0.05)。结论:热缺血再灌注之肝脏通过上调B71和B72的表达增加了肝脏的免疫原性。
Objective：To Investigate the expression of costlmulatory molecule B7-1 and B7-2 by warm ischemia/reperfusion（1/R）injury In rat liver and the meening of ImmunolosY. Methods ： Male Wistar rats were randomly dlvicted Into 3 groups：A（ischemia 30 mins, referfusion 24h）, B（ischemia 60 mins,referfusion 24h）,C（sham operation group）. A model of hepatic warm I/R in rats was establlshed according to the method of Ohmorid. The expression of B7-1 and B7-2 mRNA was analyzed by real-time reverse transcription polyrnerase chain reaction（RT-POR）, Results ：Oompared with the sham operation group,the expression of B7-1 and B7-2 mRNA increased sisnificently In the rats unclersoing I/R procedure（P〈0.01）.Conclusion：Warm ischemia/reperfusion injury may increase the Immunosenicity of the liver by upresulating the costimulatory molecule BT-1 ancl B7-2.
出处
《中国现代普通外科进展》
CAS
2006年第3期151-153,共3页
Chinese Journal of Current Advances in General Surgery
基金
山东省自然科学基金资助项目(Y2003c15)
关键词
共刺激分子
再灌注损伤
肝移植
大鼠
Wistar
Costimulatory molecule
Reperfuslon（injury）·Liver Transplantation·Rats, Wistar
