摘要
目的通过裸鼠移植瘤试验,观察NSAIDs药物对胃癌移植瘤的抑制作用;通过NSAIDs对COX-2、VEGF及NF-κB表达影响的研究,证实抑制胃癌新生血管形成在NSAIDs抗胃癌发生发展中的重要作用;探讨COX-2与核转录因子NF-κB的关系及核转录因子NF-κB在NSAIDs抗胃癌中的作用。方法应用裸鼠移植瘤实验,观察NSAIDs对胃癌移植瘤的抑制率;应用免疫组织化学方法及图象分析仪检测NSAIDs作用前后胃癌移植瘤COX-2、VEGF及NF-κB表达;结果尼美舒利的抑瘤率为85%、舒林酸的抑瘤率为88.96%、吲哚美辛抑瘤率为75%,与对照组比较有显著性差异(P<0.01);免疫组织化学及图象分析仪检测结果显示尼美舒利、舒林酸、吲哚美辛均可使COX-2、VEGF及NF-ΚB的表达下调。结论不同NSAIDs均表现有抑制胃癌裸鼠移植瘤生长的作用。NSAIDs可能通过下调VEGF表达而抑制肿瘤新生血管的生成。NF-κB表达与COX-2蛋白表达呈正相关,NSAIDs可能是通过NF-κB途径抑制COX-2表达,从而抑制胃癌细胞增殖,促进细胞凋亡;NF-κB表达与VEGF正相关,因而对VEGF可能有正面调节作用。NSAIDs可能通过影响NF-κB的活性,进而影响胃癌血管的形成,而起到抑制胃癌的作用。
Objective By tumor implantation studing, to observe the effect of NSAIDs on suppress the growth of gastric cancer xenografts, To evalutate the NSAIDs＇ effects on the expression of COX-2, VEGF, NF-κB and their association with gasfie cancer. Methods Nude mice tumor implantation was used to observe the NSAIDs effect on the growth inhibition of gastric cancer xenograsts. Immunehistochemistry, was used to detect expression of COX-2,VEGF,NF-KB.Results In tumor implantation studies,oral NSA/Ds administration could suppress the course of tumor growth more than control groups. Immunecillehemistry showed NSAIDs could down- regulate the expression of COX-2, VEGF, and.NF-κB. Conclusion NSAIDs can suppress the growth of gastric cancer xenografts, NSAIDs can inhibit the expression of COX-2 through NF-κB pathway and positive relate with VEGF. NSAIDs can reduce the formatting of gastric cancer blood vessel by influencing the activity of NF-κB. NSAIDs could, act as a chemoprevenfion and treatment drug of gastric cancer,
出处
《中国实验诊断学》
2006年第6期575-577,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅科研资助项目(20010707)
