摘要
目的:观察给予不同剂量碘对小鼠特异性免疫和非特异性免疫功能的变化,探讨高剂量碘对免疫功能的影响。方法:用160只BaLB/c雌性小鼠,按体重随机分4组,每组40只,在自来水中加入不同剂量碘酸钾,使其含碘1500、3000、6000μg/L,以自来水为对照,自由饮水,30d后测定小鼠脏体比,淋巴细胞增殖反应能力,T细胞表面主要辅助分子CD4+、CD8+比值的变化,CD4+T细胞Th1,Th2比值的变化,半数溶血值,抗体生成细胞数,NK细胞活性,腹腔巨噬细胞吞噬鸡红细胞的吞噬率、吞噬指数。结果:含碘6000μg/L组的脾脏指数、半数溶血值、抗体生成细胞数,CD4+/CD8+与正常对照组相比降低,淋巴细胞增殖反应能力、IFN-γ/IL-4、NK细胞活性与正常对照组相比升高,差异显著。腹腔巨噬细胞吞噬鸡红细胞的吞噬率、吞噬指数及胸腺指数在各剂量组间的差异无统计学意义。结论:碘与机体免疫反应相关,小鼠饮水中含碘达到6000g/L时即可造成免疫功能紊乱,机体存在自体免疫性疾病发生的危险。
Objective： To Investigate the effect of iodine on specific immunity and nonspecific immunity in mice, and the relationship between excess dosage iodine and immune system. Method： 160 female BaLB/c mice were divided into 4 groups randomly according to their body weight, 40 for each group ,10 of each group was considered as one batch for test . Mice in 4 groups were orally administrated with tap water （control） and 1 500, 3 000, 6 000μg/L iodine respectively for 30 d. They were used to observe spleen index , thymus index, lymphocyte proliferation response, CD4^＋/CD8^＋, Th1/Th2, 50% haemolysis value, antibody formation cell, phagocyte function, the activity of natural-killer cells（NK）. Results： In iodine 6 000 μg/L group, spleen index ,thymus index, 50% haemolysis value, CD4^＋/CD8^＋were significantly lower, and lymphocyte proliferation response, Th1/Th2, the activity of NK cell were significantly higher than those in control group. There were no significant differences about phagocyte function, thymus index among all dosages. Conclusion： Iodine as an exogenous chemical material is related to immune reaction, and the dosage 6 000μg/L can induce disorders of immune function in mice. It may have a central role in the development of autoimmune disease.
出处
《营养学报》
CAS
CSCD
北大核心
2006年第3期203-205,共3页
Acta Nutrimenta Sinica
基金
国家自然科学基金(No.30230330)
关键词
高剂量
碘酸钾
免疫功能
影响
excess potassium iodate
immune function
effect
