摘要
本文用LN及含其活性位点序列的合成肽段cYIGSR和RGDS对小鼠EPC细胞与LN的相互作用机制进行研究。结果表明:合成肽段cYIGSR和RGDS能促进EPC粘附并具协同效应。cYIGSR还能促进EPC扩展与次生TGCs迁移。LNA链上RGD和B_1链上YIGSR两个活性位点协同地参与了LN对EPC的粘附、扩展以及次生TGCs的迁移的促进作用。cY+R合用不能完全竞争性抑制EPC与LN的结合,说明还有其他作用位点存在。
Implantation of the mouse embryo is dependent on the interactions between the trophoblast cells and the surrounding uterine environment. The initial invasion by primary trophoblast stimulates the uterine stromal fibroblasts differentiate into deci-dual cells,which deposit a peri cellular matrix consisting of LN, FN and Col Ⅳ. The secondary trophoblast giant cells (TGCs) from ectoplacental cone (EPC) invade the decidua to form the fetal portion of the placenta. We used synthetic peptides cyclic YIGSR (cYIGSR) and RGDS to study the mechanisms of EPC cells interaction with LN. The results indicated that cYI-
GSR and RGDS promoted EPC attachment and had synergistic effect, and cYIGSR also promoted EPC outgrowth and secondary TGCs migration. LN supported EPC attachment and outgrowth, as well as secondary TGCs migration from EPC. Biologically active domains RGD of LN A chain and YIGSR of LN B, chain participated synergistically in EPC attachment, outgrowth, as well as secondary TGCs migration. Since synthetic peptides cYIGSR and RGDS can't competitively inhibit EPC attachment with LN completely, there must be other binding sites involved in the interaction.
出处
《实验生物学报》
CSCD
1996年第2期125-131,共7页
Acta Biologiae Experimentalis Sinica
基金
计划生育生殖生物学国家重点实验室资助项目
关键词
鼠
小鼠
外胎盘锥
细胞
层粘连蛋白
Ectoplacental cone. Laminin. Attachment. Outgrowth. Migration
