摘要
目的 探讨纳洛酮在心肌缺血再灌注损伤时对血浆内皮素-1（ET-1）变化的影响和对心肌超微结构的保护。方法 新西兰兔20只。随机分成2组（缺血再灌注组、纳洛酮保护组），每组10只，制作心肌缺血再灌注损伤模型，分别于结扎前、结扎后30min松开结扎线恢复灌流时及灌注后0．5、1、2,4、6h采血，测定ET-1含量，在6h后分别取缺血部位的心肌标本，用电镜观察心肌超微结构的变化。结果心肌缺血再灌注损伤后0．5～1h ET-1含量明显高于结扎前（P（0．05或P（0．01）；纳洛酮保护组ET-1含域均显著低于结扎前（P〈0．05或P（0．01）。超微结构改变：心肌缺血再灌注组，大部分心肌纤维呈收缩状态，大部分肌原纤维局灶性溶解、断裂，核周水肿；纳洛酮保护组，肌原纤维结构较清晰，排列整齐，未见明显断裂，肌原纤维收缩状态缓解，线粒体水肿减轻，胞浆轻度水肿。结论 纳洛酮可有效抑制心肌缺血再灌注损伤时ET-1的合成和分泌，减轻ET-1对血管和心肌组织的损伤作用；并对心肌超微结构的改变有明显保护作用，心肌细胞超微结构损伤明显减轻。
Objective To study the effects of naloxone on Endothelin - 1 （ ET - 1 ） in plasma and myocardial ultrastructure during myocardial ischemia- reperfusion （I/R） injury in rabbits. Methods Using myocardial ischemia medels and myocardial ischemia- reperfusion injury models in rabbits that were made by means of ligating the left anterior descending branch of coronary artery, to investigate the change of ET- 1 in plasma during I/R injury, and after the protection with naloxone, an antagonist of opiate receptor. 20 New Zealand rabbits were randomly assigned to 2 groups （each 10 rabbits in naloxone protection and ischemia - reperfusion group）. The blood was taken at different time in each group. The blood samples were taken before ligation,at 30 minutes later after loosening the ligation, and at 0.5h, lh, 2h, 4h, 6h after reperfusion, contents of ET - 1 was measured , the samples of myocardial ischemia were taken, the changes of myocardial ultrastructure were observed through electron microscope. Results For ischemia - reperfusion group, the level of ET- 1 increased and reached the peak after 4h. The contents of ET 1 at 0. 5h and 1 h after reperfusion were much higher than before ischemia （ P 〈 0. 05 or P 〈 0.01 ）. For naloxone protection group, the content of ET - 1 was much lower than before ischemia （ P 〈 0. 05 or P 〈 0, 01 ）. Far ischemia - reperfusion group, most of cardiac muscles were in the contracting state, the myofibril was contracted significantly, some was dissolved and broke locally. The edema was found under the myolemma, kytoplasm and mitochondria mad the bubbles of mltochondria passed though the stroma. And the edema was found on the circumference of nucleus. For the protection group, the structure of myofibril was clear and its arrangement was in good order, no obvious breakage was found. The contracting of myofibril, the edema of mitochondria and kytoplasm were moderated. Conclusion Naloxone may effectively control the synthesis and secreting of ET- 1 in plasma, reduce the in
出处
《浙江临床医学》
2006年第6期564-565,共2页
Zhejiang Clinical Medical Journal
基金
温州市科技局基金资助项目(S2000A23)
关键词
纳洛酮
再灌注损伤
ET-1
超微结构
Naloxone myocardial ischemia- reperfusion （I/R） injury ET- 1 Myocardial ultrastructure
