摘要
目的探讨基因重组人生长素(recomb inant hum an growth hormone,rhGH)对创伤性关节软骨创伤的修复作用。方法选用大耳白兔25只,随机分成5组,每组5只。在两侧髌股关节股骨侧的关节面中心分别造成直径3.5 mm的全层关节软骨缺损。选择右侧为实验组,自术后起,膝关节内注射0.1 U/kg的r-hGF,每周3次,共4周;左侧为对照组,与实验组同一时间注射等容量生理盐水。于术后4、6、8、12、24周分别取材进行大体、光镜和透射电镜观察。结果实验组修复组织填充快,质量好。4周时缺损区充填完全浅部,再生软骨明显深部纤维组织为主。24周时再生组织与周围正常软骨外观相近,肉眼难以区分;光镜下损伤区基本恢复正常软骨组织结构;电镜下可见到大量,形态成熟为主的软骨细胞。而生理盐水对照组6周时缺损区仍有浅表凹陷,并以纤维组织性修复为主。除术后4周外,其余各期两组的组织学评分均有显著性差异(P<0.01)。实验同时显示再生软骨于24周后变薄。3例修复软骨较正常薄,2例修复软骨与正常软骨厚度大致相同均无裂隙形成。结论rhGF局部关节腔内注射,能促进创伤性关节软骨缺损的修复,为微创性修复关节软骨缺损提供了一条新途径。
Objective To investigate the mechanism of recombinant human growth hormone （rhGH） in articular cartilage defect repair in vivo. Methods The cylindrical, full-thick articular cartilage defects, 3.5 mm in diameter, were made in the knee joints of 25 rabbits, rhGH of 0.1 U/kg was injected into the right knee joints 3 times a week for 4 weeks and equal volume of physiological saline to the left knee joints as control. The animals were killed respectively in 4, 6, 8, 12, 24 weeks, and the macroscopic, histologic and ultrastractural examinations were performed. Results The repairing process in experimental group was faster and better than that in the control. In the experimental groups, the defect was filled on week 4, the regenerated cartilage mainly in the shallow region and fibrous tissue in the deep region; on week 24, the regenerated tissue was morphologically close to the neighbour normal cartilage, hard to differentiate by macroscopy; Light microscopy showed the defect recovered to normal cartilage structure and the electron microscopy showed a large quantity of mature cartilage cells. In the control groups, the defect was of shallow introcession, mainly of fibrous tissue. The histological examination showed the significant difference between experimental and control groups （P 〈 0. 01 ）, except for the results on week 4. The regenerated cartilage cartilage in 3 cases, close to normal cartilage without fissuration in got thinner on week 24, thinner than normal 2 cases. Conclusion rhGH plays an important role in cartilage metabolism, effectively facilitates the repair of articular cartilage defect and provides a new non-traumatic method for the treatment of articular cartilage defects.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第13期1417-1419,共3页
Journal of Third Military Medical University
关键词
人生长素
软骨
组织学
human growth hormone
cartilage
histology
