摘要
目的研究凋亡信号调节激酶(apoptosis signal regu lating k inase 1,ASK1)在高血压心脏肥大中的作用。方法复制腹主动脉缩窄高血压大鼠模型,用比色法测定假手术组和高血压组左心室活性氧(reactive oxygen spec ies,ROS)水平,用免疫印迹法测定ASK1蛋白表达。结果高血压大鼠左心室质量指数、ROS水平、ASK1蛋白表达均显著高于假手术组(P<0.05)。结论压力超负荷下随着ROS的升高,激活的ASK1可能在心脏肥大信号传导途径中发挥重要作用。
Objective To study the effect of apoptosis signal regulating kinase 1 （ASK1） protein expression in hypertrophic myocardium in rat models of hypertension. Methods The rat model of hypertension was established by abdominal aortic constriction. Six weeks later, the reactive oxygen species （ROS） level and ASK1 protein expression in myocardium of left ventricle were detected respectively by color matching and Western blotting. Results The left ventricular mass index, ROS level and ASK1 protein expression all increased significantly in hypertensive rats as compared with those underwent sham operation. Conclusion Under chronic pressure overload, the activated ASK1 with the increasing ROS may play an important role in signal transduction pathway of myocardial hypertrophy.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第12期1314-1316,共3页
Journal of Third Military Medical University
关键词
高血压
心脏肥大
活性氧
凋亡信号调节激酶
hypertension
myocardial hypertrophy
reactive oxygen species
apoptosis signal regulating kinase 1
