摘要
目的通过体外细胞培养和建立大鼠胆管结扎(b ile duct ligation,BDL)所致阻塞性胆汁淤积动物模型,在蛋白水平观察多耐药相关蛋白MRP3(mu ltidrug resistance-assoc iated prote in 3,MRP3)和核受体RXRα(retinoid-X receptor al-pha,RXRα)表达的关系。方法用鹅去氧胆酸(chenodeoxycholic ac id,CDCA)或苯巴比妥(phenobarb ital,PB)分别刺激培养的肝癌细胞HepG2并建立DBL阻塞性胆汁淤积大鼠模型后,分别抽提HepG2细胞总蛋白、核蛋白和大鼠肝脏细胞膜蛋白和核蛋白,W estern b lot方法检测MRP3和RXRα蛋白表达水平的变化。结果在细胞水平,CDCA和PB可诱导HepG2细胞膜MRP3蛋白表达增高,并抑制细胞核RXRα蛋白表达;在动物体内,BDL大鼠肝脏MRP3明显增加,同时RXRα表达明显下降。结论肝细胞膜蛋白MRP3表达的上调可能与核受体RXRα表达抑制有关。
Objective To investigate the relationship between the hepatic membrane protein MRP3 （multidrug resistance-associated protein 3, MRP3 ） expression and the nuclear hormone receptor RXRα （retinoid-X receptor alpha, RXRα）expression in both cultured hepatoma cell HepG2 and bile duct ligated （BDL） rat liver. Methods Total cellular protein and nuclear protein were isolated from HepG2 cells induced by chenodeoxycholic acid （CDCA） or phenobarbital （PB）, as well as from the liver tissue of BDL rats. The protein expressions of MRP3 and RXRα were determined by Western blotting. Results The membrane protein MRP3 expression was significantly enhanced and the nuclear receptor RXRα expression was suppressed by CDCA or PB in HepG2 cells. Similarly up-regulation of MRP3 and down-regulation of RXRα were also observed in BDL rat liver. Conclusion The up-regulation of hepatic membrane protein MRP3 may be associated with down-regulation of nuclear receptor RXRα.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第12期1298-1301,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(30570842)~~
