摘要
用反向PCR结合计算机图象处理技术检测卡托普利(CaP)对培养家兔主动脉平滑肌细细内皮素-1(ET-1)mRNA表达的影响。扩增的ET-1cDNA为425bp,经HindⅢ酶切后为279bg和146bg。血管紧张素Ⅱ(Ang-Ⅱ)引起ET-1mRNA表达,诱导表达有时间依赖性(作用3h,表达明显增加,作用5h,表达到高峰,作用7h,表达开始下降)。CaP(1和10μmolL^(-1))明显抑制Ang-Ⅱ引起的ET-1mRNA表达,抑制分别达73％和84％。这些结果表明CaP能直接抑制血管平滑肌细胞的ET—1基因表达。
To investigate the effect of captopril (Cap) on expression of endothelin-l (ET-l) mRNA in rabbit aortic smooth muscle cells using RT-PCR coupled with digital image processing techniques, a 425bp ET-1 cDNA was synthesized by RT-PCR. Two fragments of 425bp cDNA (279 bp and 146 bp) were obtained by HindⅢ enzymatic dissociation. Angiotensin Ⅱ (Ang-Ⅱ) induced ET-1 mRNA expression. Induction of ET-1 mRNA expression was exhibited transient kinetics (marked increase at 3 h, peak at 5h, decline at 7h). Cap (1 or 10 μmol-1) markedly inhibited the induction of ET-l mRNA expression by Ang-I (73% and 84%). These results showed that Cap Inhibited ET-1 gene expression in vascular smooth muscle cell directly.
出处
《细胞生物学杂志》
CSCD
1996年第2期82-86,共5页
Chinese Journal of Cell Biology
关键词
卡托普利
内皮素
基因表达
血管平滑肌细胞
Endothelin Gene expression Angiotensin Ⅱ Captopril Vascular smooth muscle ceU culture
