摘要
目的:研究1,25二羟维生素D3[1,25(OH)2D3]对Lscc02人喉鳞状细胞癌细胞系增殖及凋亡的影响。方法:将不同浓度的1,25(OH)2D3与Lscc02人喉鳞状细胞癌细胞系共同培养,采用MTT法、流式细胞仪及透射电镜观察该细胞系的增殖及凋亡诱导情况。结果:(10-10～10-7)mol/L浓度的1,25(OH)2D3均能抑制Lscc02细胞系的增殖,抑制作用随1,25(OH)2D3浓度的增加而增强,在半抑制浓度时,抑制作用随时间延长而增强。流式细胞仪显示,经1,25(OH)2D3作用的喉鳞状细胞癌细胞位于细胞周期G0/G1期的细胞数量明显增多且细胞凋亡数目明显增加。结论:1,25(OH)2D3体外能抑制Lscc02细胞系的增殖并诱导细胞凋亡,为临床探索1,25(OH)2D3治疗喉癌提供了重要的实验依据。
Objective：The present study was to investigate the effect of 1,25（OH）2D3 on the proliferation and apoptosis of Lscc-02 laryngeal squamous cell carcinoma cell line. Method： Lscc-02 human laryngeal squamous cell carcinoma cell line was treated with 1,25 （OH）2 D3. Cell proliferation was evaluated by MTT assay, distribution of cell cycle and rate of apoptosis determined by flow cytometry, and cell morphology and structure observed by microscopy. Result： The results showed that（10-^10- 10^-7 ） mol/L 1, 25 （OH）2 D3 inhibited the proliferation of Lscc- 02 cell line, and the inhibitory rate was positively associated with concentration of 1,25（OH）2D3 and treatment time. Cell cycle arrest in G0/G1 phase and induction of apoptosis occurred after 1,25（OH）2D3. Conclusion： 1,25 （OH）2D3 can inhibit proliferation and induce apoptosis in Lscc-02 laryngeal squamous cell carcinoma cell line in vitro, which suggests that 1,25（OH）2 D3 will be potential for the therapy of laryngeal squamous cell carcinoma.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第12期538-540,544,共4页
Journal of Clinical Otorhinolaryngology
关键词
1
25二羟维生素D3
喉肿瘤
癌
鳞状细胞
细胞增殖
细胞凋亡
1,25-dihydroxyvitamin D3
Laryngeal neoplasms
Carcinoma, squamous cell
Proliferation
Apoptosis
