摘要
杜氏盐藻(Dunaliella salina)有着极强的耐盐碱性.盐藻在低pH值条件下,主要依靠质膜上的Na+/H+逆向转运蛋白实现Na+的外排.在pH值较高的环境中,由于Na+/H+逆向转运蛋白不能够很好的发挥效能,所以盐藻质膜上很可能存在着不依赖于跨膜质子电化学势梯度的排钠机制.通过将盐藻培养液的pH值稳定在7.1和8.9,比较Na+/H+逆向转运蛋白的特异性抑制剂LiCl,以及P型ATPase的特异性抑制剂钒酸钠对盐藻生长的影响,推测盐藻质膜上存在着由不依赖于跨膜质子电化学势梯度的P型ATPase所介导的排钠机制.
The unicellular marine alga Dunaliella salina can grow in media of both high salinity and high alkalinity. At low pH, active Na^＋ -export is carried out by means of a plasma membrane localized Na^＋/H^＋ antiporter. At high alkalinity, the Na^＋/H^＋ antiporter is inefficient. For these reasons, in the plasmamembrane of the D. salina, there may be other Na^＋ -export systems which are not dependent on the electrochemical gradient generated by the plasma membrane H^＋ -ATPase. In this paper, the alga was cultured in the media of pH 7.1 and pH 8.9, then.effects of LiCl which specific inhibits Na^＋/H^＋ antiporter and orthvanadate which specific inhibits P-type ATPase were analyzed. According to the results, alternative mechanisms for Na^＋ -export are discussed . These mechanisms are plasma membrane localized and not dependent on H^＋ -gradient.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期677-681,共5页
Journal of Sichuan University(Natural Science Edition)
基金
四川省科技厅重点项目(01NG018-01)
关键词
杜氏盐藻
钒酸钠
氯化锂
排钠机制
Dunaliella salina
orthvanadate
LiCI
Na^＋ -export mechanism
